Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors

D. W.K. Ng, C. Zhang, M. Miller, Z. Shen, S. P. Briggs, Z. J. Chen

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Autopolyploidy and allopolyploidy are common in many plants and some animals. Rapid changes in genomic composition and gene expression have been observed in both autopolyploids and allopolyploids, but the effects of polyploidy on proteomic divergence are poorly understood. Here, we report quantitative analysis of protein changes in leaves of Arabidopsis autopolyploids and allotetraploids and their progenitors using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with mass spectrometry. In more than 1000 proteins analyzed, the levels of protein divergence were relatively high (∼18%) between Arabidopsis thaliana and Arabidopsis arenosa, relatively low (6.8%) between an A. thaliana diploid and autotetraploid and intermediate (∼8.3 and 8.2%) in F 1- and F 8-resynthesized allotetraploids relative to mid-parent values, respectively. This pattern of proteomic divergence was consistent with the previously reported gene expression data. In particular, many non-additively accumulated proteins (61-62%) in the F 1 and F 8 allotetraploids were also differentially expressed between the parents. The differentially accumulated proteins in functional categories of abiotic and biotic stresses were overrepresented between an A. thaliana autotetraploid and diploid and between two Arabidopsis species, but not significantly different between allotetraploids and their progenitors. Although the trend of changes is similar, the percentage of differentially accumulated proteins that matched previously reported differentially expressed genes was relatively low. Western blot analysis confirmed several selected proteins with isoforms the cumulative levels of which were differentially expressed. These data suggest high protein divergence between species and rapid changes in post-transcriptional regulation and translational modifications of proteins during polyploidization.

Original languageEnglish (US)
Pages (from-to)419-430
Number of pages12
JournalHeredity
Volume108
Issue number4
DOIs
StatePublished - Apr 2012

Bibliographical note

Funding Information:
We thank James A Birchler for the fluorescence in situ hybridization (FISH) cytogenetics workshop where CZ adapted the FISH methodology for this study and Angela Bardo in the Core Facility of Institute for Cellular and Molecular Biology at The University of Texas at Austin for assistance with fluorescence image analysis. We also thank Xiaoli Shi for helpful discussions to improve statistical analysis of the proteomic data. This study was supported by grants from the Plant Genome Research Program of the National Science Foundation (IOS0733857 to ZJC and ISO0924023 to SPB).

Keywords

  • Arabidopsis
  • gene expression
  • hybrids
  • iTRAQ
  • polyploidy
  • proteome

Fingerprint Dive into the research topics of 'Proteomic divergence in Arabidopsis autopolyploids and allopolyploids and their progenitors'. Together they form a unique fingerprint.

Cite this