Proteomic analysis of protein phosphorylation and ubiquitination in Alzheimer's disease

Stefani N. Thomas, Diane Cripps, Austin J. Yang

Research output: Chapter in Book/Report/Conference proceedingChapter

23 Scopus citations


Posttranslational modifications such as phosphorylation and ubiquitination serve, independently or together, as gatekeepers of protein transport and turnover in normal and disease physiologies. Aberrant protein phosphorylation is one of the defining pathological hallmarks of more than 20 different neurodegenerative disorders, including Alzheimer's disease (AD). The disruption of the phosphorylation of neurotransmitter receptors has been implicated as one of the causal factors of impaired memory function in AD (1-3). Another feature of AD is the aberrant accumulation of proteins that are normally degraded by the ubiquitin proteasome system upon being conjugated to ubiquitin. Thus, elucidating the protein targets of phosphorylation and ubiquitination that can serve as AD biomarkers will aid in the development of effective therapeutic approaches to the treatment of AD. This chapter provides details pertaining to the qualitative and quantitative liquid chromatography tandem mass spectrometry-based analysis of an affinity purified, phosphorylated, and ubiquitinated protein, paired-helical filament tau.

Original languageEnglish (US)
Title of host publicationNeuroproteomics
Subtitle of host publicationMethods and Protocols
EditorsAndrew Ottens, Kevin Wang, Kevin Wang
Number of pages13
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Alzheimer's disease (AD)
  • Liquid chromatography tandem mass spectrometry (LC-MS/MS)
  • Phosphorylation
  • Selected reaction monitoring (SRM)
  • Tau
  • Ubiquitin


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