Protein phosphokinase activity of rat liver nuclear membrane

Randolph C. Steer; Michael J. Wilson; Khalil Ahmed

doi:10.1016/0014-4827(79)90372-0

Protein phosphokinase activity of rat liver nuclear membrane

Randolph C. Steer
, Michael J. Wilson
, Khalil Ahmed

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The presence of protein phosphokinase activity in a purified nuclear-membrane preparation from adult rat liver was demonstrated by measuring the incorporation of ³²P from γ-³²P-ATP into endogenous nuclear-membrane proteins as well as into the exogenous protein substrates, dephosphophosvitin (DPV) and lysine-rich histone (LRH). The activity of this enzyme toward DPV was 60 times greater than that toward LRH. cAMP and cGMP did not appear to affect the phosphorylation of endogenous-membrane proteins.

Original language	English (US)
Pages (from-to)	403-406
Number of pages	4
Journal	Experimental Cell Research
Volume	119
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(79)90372-0
State	Published - Mar 15 1979

Bibliographical note

Funding Information:
This study was supported by grants from the Swedish Medical Research Council No. 04480 and 02235.

Publisher link

10.1016/0014-4827(79)90372-0

Find It

Find It at U of M Libraries

Cite this

@article{03b2b9195593440980180a790a01326e,

title = "Protein phosphokinase activity of rat liver nuclear membrane",

abstract = "The presence of protein phosphokinase activity in a purified nuclear-membrane preparation from adult rat liver was demonstrated by measuring the incorporation of 32P from γ-32P-ATP into endogenous nuclear-membrane proteins as well as into the exogenous protein substrates, dephosphophosvitin (DPV) and lysine-rich histone (LRH). The activity of this enzyme toward DPV was 60 times greater than that toward LRH. cAMP and cGMP did not appear to affect the phosphorylation of endogenous-membrane proteins.",

author = "Steer, \{Randolph C.\} and Wilson, \{Michael J.\} and Khalil Ahmed",

note = "Funding Information: This study was supported by grants from the Swedish Medical Research Council No. 04480 and 02235.",

year = "1979",

month = mar,

day = "15",

doi = "10.1016/0014-4827(79)90372-0",

language = "English (US)",

volume = "119",

pages = "403--406",

journal = "Experimental Cell Research",

issn = "0014-4827",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Protein phosphokinase activity of rat liver nuclear membrane

AU - Steer, Randolph C.

AU - Wilson, Michael J.

AU - Ahmed, Khalil

N1 - Funding Information: This study was supported by grants from the Swedish Medical Research Council No. 04480 and 02235.

PY - 1979/3/15

Y1 - 1979/3/15

N2 - The presence of protein phosphokinase activity in a purified nuclear-membrane preparation from adult rat liver was demonstrated by measuring the incorporation of 32P from γ-32P-ATP into endogenous nuclear-membrane proteins as well as into the exogenous protein substrates, dephosphophosvitin (DPV) and lysine-rich histone (LRH). The activity of this enzyme toward DPV was 60 times greater than that toward LRH. cAMP and cGMP did not appear to affect the phosphorylation of endogenous-membrane proteins.

AB - The presence of protein phosphokinase activity in a purified nuclear-membrane preparation from adult rat liver was demonstrated by measuring the incorporation of 32P from γ-32P-ATP into endogenous nuclear-membrane proteins as well as into the exogenous protein substrates, dephosphophosvitin (DPV) and lysine-rich histone (LRH). The activity of this enzyme toward DPV was 60 times greater than that toward LRH. cAMP and cGMP did not appear to affect the phosphorylation of endogenous-membrane proteins.

UR - https://www.scopus.com/pages/publications/0018394929

UR - https://www.scopus.com/pages/publications/0018394929#tab=citedBy

U2 - 10.1016/0014-4827(79)90372-0

DO - 10.1016/0014-4827(79)90372-0

M3 - Article

C2 - 570930

AN - SCOPUS:0018394929

SN - 0014-4827

VL - 119

SP - 403

EP - 406

JO - Experimental Cell Research

JF - Experimental Cell Research

IS - 2

ER -

Protein phosphokinase activity of rat liver nuclear membrane

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

Cite this