Protein nitration with aging in the rat semimembranosus and soleus muscles

Nicole A. Fugere, Deborah A. Ferrington, La Dora V. Thompson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

On the basis of the accelerated age-related effects in type II muscle, we hypothesized that with aging the semimembranosus (type II) muscle would accumulate a greater amount of oxidized proteins compared to proteins in the soleus (type I) muscle. In this study, 3-nitrotyrosine (3-NT) was used as a stable marker of protein oxidative damage. The presence of 3-NT was evaluated in muscles from young adult, old, and very old Fischer 344 rats to provide an indication of the time course of muscle protein oxidative damage. A significant age-associated increase in nitrotyrosine-modified proteins was observed. The modified proteins identified by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry include the sarcoplasmic reticulum Ca+2-ATPase, aconitase, β-enolase, triosephosphate isomerase, and carbonic anhydrase UL These proteins, involved in metabolism and calcium homeostasis, exhibited an age-dependent increase in 3-NT content in both muscles. However, significant levels of 3-NT modification were present at an earlier age in the semimembranosus muscle.

Original languageEnglish (US)
Pages (from-to)806-812
Number of pages7
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume61
Issue number8
DOIs
StatePublished - Aug 2006

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Skeletal Muscle
Muscles
Proteins
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Triose-Phosphate Isomerase
Aconitate Hydratase
Carbonic Anhydrases
Phosphopyruvate Hydratase
Muscle Proteins
Inbred F344 Rats
Hamstring Muscles
Young Adult
Mass Spectrometry
Lasers
Homeostasis
3-nitrotyrosine
Calcium

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Protein nitration with aging in the rat semimembranosus and soleus muscles. / Fugere, Nicole A.; Ferrington, Deborah A.; Thompson, La Dora V.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 61, No. 8, 08.2006, p. 806-812.

Research output: Contribution to journalArticle

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