Protein nitration with aging in the rat semimembranosus and soleus muscles

Nicole A. Fugere, Deborah A. Ferrington, La Dora V. Thompson

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47 Scopus citations


On the basis of the accelerated age-related effects in type II muscle, we hypothesized that with aging the semimembranosus (type II) muscle would accumulate a greater amount of oxidized proteins compared to proteins in the soleus (type I) muscle. In this study, 3-nitrotyrosine (3-NT) was used as a stable marker of protein oxidative damage. The presence of 3-NT was evaluated in muscles from young adult, old, and very old Fischer 344 rats to provide an indication of the time course of muscle protein oxidative damage. A significant age-associated increase in nitrotyrosine-modified proteins was observed. The modified proteins identified by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry include the sarcoplasmic reticulum Ca+2-ATPase, aconitase, β-enolase, triosephosphate isomerase, and carbonic anhydrase UL These proteins, involved in metabolism and calcium homeostasis, exhibited an age-dependent increase in 3-NT content in both muscles. However, significant levels of 3-NT modification were present at an earlier age in the semimembranosus muscle.

Original languageEnglish (US)
Pages (from-to)806-812
Number of pages7
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Issue number8
StatePublished - Aug 2006

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS This work was supported by National Institute on Aging Grants AG-17768 and AG-21626 (to L. V. Thompson).


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