Protein kinase C regulates both production and secretion of interleukin 2

Jaime F. Modiano, Rebecca Kolp, Roberta J. Lamb, Peter C. Nowell

Research output: Contribution to journalArticle

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Abstract

Inhibiting protein kinase C (PKC) activity abrogated interleukin 2 (IL2) production by mitogen-stimulated human T lymphocytes. This effect was due partially to a 50% decrease in IL2 gene expression. However, when PKC inhibitors were added after IL2 gene transcription had already proceeded for 3-4 h, the IL2 in the culture supernatants was still reduced by 30-80%, and intracellular IL2 was increased by up to 50%. The inhibition of PKC affected the expression of IL2 receptors by these cells differently; it had little effect on gene expression or on the membrane-bound form of the receptor, but it decreased soluble receptors in the supernatants by 50-80%. These data indicate that in addition to its previously defined role in gene expression, PKC can also regulate extracellular secretion of proteins critical for T cell proliferation.

Original languageEnglish (US)
Pages (from-to)10552-10561
Number of pages10
JournalJournal of Biological Chemistry
Volume266
Issue number16
StatePublished - Aug 16 1991

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Protein Kinase C
Interleukin-2
Gene expression
T-cells
Gene Expression
T-Lymphocytes
Protein C Inhibitor
Interleukin-2 Receptors
Cell proliferation
Transcription
Protein Kinase Inhibitors
Mitogens
Proteins
Genes
Cell Proliferation
Membranes

Cite this

Protein kinase C regulates both production and secretion of interleukin 2. / Modiano, Jaime F.; Kolp, Rebecca; Lamb, Roberta J.; Nowell, Peter C.

In: Journal of Biological Chemistry, Vol. 266, No. 16, 16.08.1991, p. 10552-10561.

Research output: Contribution to journalArticle

Modiano, JF, Kolp, R, Lamb, RJ & Nowell, PC 1991, 'Protein kinase C regulates both production and secretion of interleukin 2', Journal of Biological Chemistry, vol. 266, no. 16, pp. 10552-10561.
Modiano, Jaime F. ; Kolp, Rebecca ; Lamb, Roberta J. ; Nowell, Peter C. / Protein kinase C regulates both production and secretion of interleukin 2. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 16. pp. 10552-10561.
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