Protein kinase C regulates both production and secretion of interleukin 2

Jaime F. Modiano, Rebecca Kolp, Roberta J. Lamb, Peter C. Nowell

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Inhibiting protein kinase C (PKC) activity abrogated interleukin 2 (IL2) production by mitogen-stimulated human T lymphocytes. This effect was due partially to a 50% decrease in IL2 gene expression. However, when PKC inhibitors were added after IL2 gene transcription had already proceeded for 3-4 h, the IL2 in the culture supernatants was still reduced by 30-80%, and intracellular IL2 was increased by up to 50%. The inhibition of PKC affected the expression of IL2 receptors by these cells differently; it had little effect on gene expression or on the membrane-bound form of the receptor, but it decreased soluble receptors in the supernatants by 50-80%. These data indicate that in addition to its previously defined role in gene expression, PKC can also regulate extracellular secretion of proteins critical for T cell proliferation.

Original languageEnglish (US)
Pages (from-to)10552-10561
Number of pages10
JournalJournal of Biological Chemistry
Volume266
Issue number16
StatePublished - 1991

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