Protein Kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) gene polymorphisms and Diabetic Retinopathy in a south Indian cohort

Satagopan Uthra; Rajiv Raman; Bickol N. Mukesh; Samuel A. Rajkumar; Padmaja Kumari R; Praveena Lakshmipathy; Perumal Gnanamoorthy; Tarun Sharma; Catherine A. McCarty; Govindasamy Kumaramanickavel

doi:10.3109/13816810903426231

Protein Kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) gene polymorphisms and Diabetic Retinopathy in a south Indian cohort

Satagopan Uthra, Rajiv Raman, Bickol N. Mukesh, Samuel A. Rajkumar, Padmaja Kumari R, Praveena Lakshmipathy, Perumal Gnanamoorthy, Tarun Sharma, Catherine A. McCarty, Govindasamy Kumaramanickavel

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Abstract

Purpose: Polymorphisms in protein kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) genes have been associated with diabetic nephropathy and retinopathy respectively. Association of promoter polymorphisms1504C/T and1440G/T in PRKCB1 gene and sequence variations in exon 4 of PEDF gene are studied with diabetic retinopathy (DR) in a south Indian population based cohort. Methods: Type 2 diabetic patients with and without retinopathy (DR and DR- respectively) were recruited. The promoter region of PRKCB1 gene and exon 4 of PEDF genes were sequenced by polymerase chain reaction based direct sequencing and their frequencies were analyzed using relevant statistical tests. Results: The genotype and alleles of the two promoter polymorphisms of PRKCB1 gene were uniformly distributed among DR and DR- and hence were not associated with the disease. The haplotypes were also not significantly associated with DR. A T130T polymorphism observed in the PEDF gene showed modest association with absence of diabetic retinopathy. Conclusion: Our results suggest lack of association of PRKCB1 gene promoter polymorphisms and moderate protective association of PEDF gene polymorphism with DR in the south Indian population.

Original language	English (US)
Pages (from-to)	18-23
Number of pages	6
Journal	Ophthalmic Genetics
Volume	31
Issue number	1
DOIs	https://doi.org/10.3109/13816810903426231
State	Published - Mar 1 2010

Keywords

Diabetic retinopathy
PEDF
PRKCB1
South India
Type 2 diabetes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/13816810903426231

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Uthra, S., Raman, R., Mukesh, B. N., Rajkumar, S. A., Kumari R, P., Lakshmipathy, P., Gnanamoorthy, P., Sharma, T., McCarty, C. A., & Kumaramanickavel, G. (2010). Protein Kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) gene polymorphisms and Diabetic Retinopathy in a south Indian cohort. Ophthalmic Genetics, 31(1), 18-23. https://doi.org/10.3109/13816810903426231

Uthra, S, Raman, R, Mukesh, BN, Rajkumar, SA, Kumari R, P, Lakshmipathy, P, Gnanamoorthy, P, Sharma, T, McCarty, CA & Kumaramanickavel, G 2010, 'Protein Kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) gene polymorphisms and Diabetic Retinopathy in a south Indian cohort', Ophthalmic Genetics, vol. 31, no. 1, pp. 18-23. https://doi.org/10.3109/13816810903426231

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abstract = "Purpose: Polymorphisms in protein kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) genes have been associated with diabetic nephropathy and retinopathy respectively. Association of promoter polymorphisms1504C/T and1440G/T in PRKCB1 gene and sequence variations in exon 4 of PEDF gene are studied with diabetic retinopathy (DR) in a south Indian population based cohort. Methods: Type 2 diabetic patients with and without retinopathy (DR and DR- respectively) were recruited. The promoter region of PRKCB1 gene and exon 4 of PEDF genes were sequenced by polymerase chain reaction based direct sequencing and their frequencies were analyzed using relevant statistical tests. Results: The genotype and alleles of the two promoter polymorphisms of PRKCB1 gene were uniformly distributed among DR and DR- and hence were not associated with the disease. The haplotypes were also not significantly associated with DR. A T130T polymorphism observed in the PEDF gene showed modest association with absence of diabetic retinopathy. Conclusion: Our results suggest lack of association of PRKCB1 gene promoter polymorphisms and moderate protective association of PEDF gene polymorphism with DR in the south Indian population.",

keywords = "Diabetic retinopathy, PEDF, PRKCB1, South India, Type 2 diabetes",

author = "Satagopan Uthra and Rajiv Raman and Mukesh, {Bickol N.} and Rajkumar, {Samuel A.} and {Kumari R}, Padmaja and Praveena Lakshmipathy and Perumal Gnanamoorthy and Tarun Sharma and McCarty, {Catherine A.} and Govindasamy Kumaramanickavel",

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doi = "10.3109/13816810903426231",

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T1 - Protein Kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) gene polymorphisms and Diabetic Retinopathy in a south Indian cohort

AU - Uthra, Satagopan

AU - Raman, Rajiv

AU - Mukesh, Bickol N.

AU - Rajkumar, Samuel A.

AU - Kumari R, Padmaja

AU - Lakshmipathy, Praveena

AU - Gnanamoorthy, Perumal

AU - Sharma, Tarun

AU - McCarty, Catherine A.

AU - Kumaramanickavel, Govindasamy

PY - 2010/3/1

Y1 - 2010/3/1

N2 - Purpose: Polymorphisms in protein kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) genes have been associated with diabetic nephropathy and retinopathy respectively. Association of promoter polymorphisms1504C/T and1440G/T in PRKCB1 gene and sequence variations in exon 4 of PEDF gene are studied with diabetic retinopathy (DR) in a south Indian population based cohort. Methods: Type 2 diabetic patients with and without retinopathy (DR and DR- respectively) were recruited. The promoter region of PRKCB1 gene and exon 4 of PEDF genes were sequenced by polymerase chain reaction based direct sequencing and their frequencies were analyzed using relevant statistical tests. Results: The genotype and alleles of the two promoter polymorphisms of PRKCB1 gene were uniformly distributed among DR and DR- and hence were not associated with the disease. The haplotypes were also not significantly associated with DR. A T130T polymorphism observed in the PEDF gene showed modest association with absence of diabetic retinopathy. Conclusion: Our results suggest lack of association of PRKCB1 gene promoter polymorphisms and moderate protective association of PEDF gene polymorphism with DR in the south Indian population.

AB - Purpose: Polymorphisms in protein kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) genes have been associated with diabetic nephropathy and retinopathy respectively. Association of promoter polymorphisms1504C/T and1440G/T in PRKCB1 gene and sequence variations in exon 4 of PEDF gene are studied with diabetic retinopathy (DR) in a south Indian population based cohort. Methods: Type 2 diabetic patients with and without retinopathy (DR and DR- respectively) were recruited. The promoter region of PRKCB1 gene and exon 4 of PEDF genes were sequenced by polymerase chain reaction based direct sequencing and their frequencies were analyzed using relevant statistical tests. Results: The genotype and alleles of the two promoter polymorphisms of PRKCB1 gene were uniformly distributed among DR and DR- and hence were not associated with the disease. The haplotypes were also not significantly associated with DR. A T130T polymorphism observed in the PEDF gene showed modest association with absence of diabetic retinopathy. Conclusion: Our results suggest lack of association of PRKCB1 gene promoter polymorphisms and moderate protective association of PEDF gene polymorphism with DR in the south Indian population.

KW - Diabetic retinopathy

KW - PEDF

KW - PRKCB1

KW - South India

KW - Type 2 diabetes

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Protein Kinase C β (PRKCB1) and pigment epithelium derived factor (PEDF) gene polymorphisms and Diabetic Retinopathy in a south Indian cohort

Abstract

Keywords

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