Protein adsorption and biomimetic mineralization behaviors of PLL-DNA multilayered films assembled onto titanium

Wenli Gao, Bo Feng, Yuxiang Ni, Yongli Yang, Xiong Lu, Jie Weng

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38 Scopus citations


Titanium and its alloys are frequently used as surgical implants in load bearing situations, such as hip prostheses and dental implants, owing to their biocompatibility, mechanical and physical properties. In this paper, a layer-by-layer (LBL) self-assembly technique, based on the polyelectrolyte- mediated electrostatic adsorption of poly-l-lysine (PLL) and DNA, was used to the formation of multilayer on titanium surfaces. Then bovine serum albumin (BSA) adsorption and biomimetic mineralization of modified surfaces were studied. The chemical composition and wettability of assembled substrates were investigated by X-ray photoelectron spectroscopy (XPS), fluorescence microscopy and water contact angle measurement, respectively. The XPS analysis indicated that the layers were assembled successfully through electrostatic attractions. The measurement with ultraviolet (UV) spectrophotometer revealed that the LBL films enhanced ability of BSA adsorption onto titanium. The adsorption quantity of BSA on the surface terminated with PLL was higher than that of the surface terminated with DNA, and the samples of TiOH/P/D/P absorbed BSA most. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) showed that samples of assembled PLL or/and DNA had better bioactivity in inducing HA formation. Thus the assembling of PLL and DNA onto the surface of titanium in turn via a layer-by-layer self-assembly technology can improve the bioactivity of titanium.

Original languageEnglish (US)
Pages (from-to)538-546
Number of pages9
JournalApplied Surface Science
Issue number2
StatePublished - Nov 1 2010

Bibliographical note

Funding Information:
This work was jointly supported by the Chinese NSFC ( 50871093 ) and FANEDD ( 200554 ).


  • Biomimetic mineralization
  • DNA
  • Layer-by-layer
  • Poly-l-lysine
  • Protein adsorption


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