We investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and Brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in male Fischer 344 rats harbouring a human intestinal microflora. We found that the four dietary components significantly reduced IQ-induced DNA damage in hepatocytes (reduction ranged from 74% with inulin to 39% with Brussels sprouts) and colonocytes (reduction ranged from 68% with inulin to 56% with Brussels sprouts). This chemoprotective effect correlated with the induction of hepatic UDP-glucuronosyl transferase following Brussels sprouts consumption, and with alterations of bacterial metabolism in the distal gut (acidification, increase of butyrate proportion, decrease of β-glucuronidase activity) following inulin consumption.
|Original language||English (US)|
|Number of pages||7|
|Journal||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences|
|State||Published - Mar 25 2004|
Bibliographical noteFunding Information:
This study was financially supported by the EC, under the specific RTD program “Quality of Life and Management of Living Resources”, QLK1-CT99-01197, entitled “Heterocyclic Amines in Cooked Foods—Role in Human Health”. The authors thank Rosa Durao for animal care, Sandrine Bruel, Solène Garrido, Françoise Popot and Pierre Vaissade for their technical help during the experiments. Many thanks also to Dr. Ruud Verkerk (University of Wageningen, The Netherlands) for the gift of freeze–dried Brussels sprouts and to Dr. Jan Van Loo (Orafti) for the RAFTILOSE ® Synergy 1.
- 2-Amino-3-methylimidazo[4,5-f] quinoline
- Fermented milk