Protective effects of Brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in the human flora associated F344 rat: Role of xenobiotic metabolising enzymes and intestinal microflora

Christèle Humblot, Evelyne Lhoste, Siegfried Knasmüller, Karine Gloux, Aurélia Bruneau, Martine Bensaada, José Durao, Sylvie Rabot, Claude Andrieux, Fekadu Kassie

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Abstract

We investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and Brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)-induced genotoxicity in male Fischer 344 rats harbouring a human intestinal microflora. We found that the four dietary components significantly reduced IQ-induced DNA damage in hepatocytes (reduction ranged from 74% with inulin to 39% with Brussels sprouts) and colonocytes (reduction ranged from 68% with inulin to 56% with Brussels sprouts). This chemoprotective effect correlated with the induction of hepatic UDP-glucuronosyl transferase following Brussels sprouts consumption, and with alterations of bacterial metabolism in the distal gut (acidification, increase of butyrate proportion, decrease of β-glucuronidase activity) following inulin consumption.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume802
Issue number1
DOIs
StatePublished - Mar 25 2004

Keywords

  • 2-Amino-3-methylimidazo[4,5-f] quinoline
  • Brassica
  • Fermented milk
  • Genotoxicity
  • Oligosaccharides

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