Protective effect of thymidine against the cytostatic action of cyclic adenosine 3',5' monophosphate in the cell cultures of mammals (Russian)

V. A. Polunovsky

Research output: Contribution to journalArticlepeer-review

Abstract

Under the action of 1 mM of cyclic adenosine 3',5' monophosphate (cAMP) the survival and rate of reproduction of culture cells of the Chinese hamster decreased, in comparison with the control group, to 27 and 42%, respectively. The addition of 0.02 mM thymidine together with cAMP almost completely eliminated the cytostatic action of the latter. Thymidine also prevented the cytostatic effect of noncyclic 5' AMR, but failed to influence the death of the cells with the action of dibutyryl cAMP and theophylline. Thymidine failed to prevent the inhibitory action of cAMP on the mutant strains of mouse cells which had been made defective by thymidine kinase. In the concentrations under study the cystostatic action of exogenous cAMP on the cells of mammals served as the sequence of splitting to 5' AMP in the culture medium and was realized by blocking one of the stages of TMP synthesis.

Original languageEnglish (US)
Pages (from-to)93-95
Number of pages3
JournalByulleten Eksperimentalnoi Biologii i Meditsiny
Volume80
Issue number7
StatePublished - Jan 1 1975

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