Protection of organ specific genotoxic effects of 2-amino-3-methylimidazo[4,5-F] quinoline (IQ) by benzyl isothiocyanate (BITC), glucotropaeolin (GT) and garden cress juice in in vivo single cell gel electrophoresis (SCGE) assay with primary rat hepatocytes and colonocytes

F. Kassie, H. M. Qin, S. Rabot, S. Knasmüller

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The single cell gel electrophoresis (SCGE) assay also known as comet assay is a relatively new genotoxicity test that detects, among other kinds of genotoxic effects, single and double strand breaks, alkali labile sites and DNA-cross linking. We employed the SCGE assay in vivo to assess genotoxic effects of IQ in organs of rats which are amenable to develop tumor upon treatment of the animals with the carcinogen. In subsequent experiments, we investigated the potential protective effects of benzyl isothiocyanate (BITC), glucotropaeolin (GT) and garden cress (GC) juice against the effects of IQ. The results showed a concordance between findings in SCGE assay and long term carcinogenicity tests in that pronounced genotoxic effects were induced in organs of rats where tumors were preferentially induced by IQ. These effects were drastically reduced upon pretreatment with BITC, GT and GC juice for three consecutive days. The antigenotoxic effect of BITC, GT, and GC juice might be attributed, at least partly, to induction of glutathione S- transferase (GST) and UDP-glucuronyl transferase (UDPGT) by these agents. In conclusion, SCGE assay was found to be sensitive enough to detect the genotoxicity of IQ and it could also be applied to identify dietary protective substances.

Original languageEnglish (US)
Pages (from-to)41-43
Number of pages3
JournalNeoplasma
Volume46
Issue numberSUPPL. 1
StatePublished - 1999

Keywords

  • Benzyl isothiocyanate
  • Garden cress juice
  • Genotoxic effects
  • Glucotropaeolin
  • IQ
  • Protective effects
  • Protective substances

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