Protection by acidotic pH against anoxia/reoxygenation injury to rat neonatal cardiac myocytes

John M. Bond, Brian Herman, John J. Lemasters

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

We assessed the effect of acidosis on cell killing during anoxia and reoxygenation in cultured rat neonatal cardiac myocytes. After 4.5 hours of anoxia and glycolytic inhibition with 2-deoxyglucose, loss of viability was >90% at pH 7.4. In contrast, at pH 6.2-7.0, viability was virtually unchanged. To model changes of pH and oxygenation during ischemia and reperfusion, myocytes were made anoxic at pH 6.2 for 4 hours, followed by reoxygenation at pH 7.4. Under these conditions, reoxygenation precipitated loss of viability to about half the cells. When pH was increased to 7.4 without reoxygenation, similar lethal injury occurred. No cell killing occurred after reoxygenation at pH 6.2. We conclude that acidosis protects against lethal anoxic injury, and that a rapid return from acidotic to physiologic pH contributes significantly to reperfusion injury to cardiac myocytes-a 'pH paradox'.

Original languageEnglish (US)
Pages (from-to)798-803
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number2
DOIs
StatePublished - Sep 16 1991

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