Prostaglandin E2 stimulates angiogenesis by activating the nitric oxide/cGMP pathway in human umbilical vein endothelial cells

Seung Namkoong, Seon Jin Lee, Chun Ki Kim, Young Mi Kim, Hun Taeg Chung, Hansoo Lee, Jeong A. Han, Kwon Soo Ha, Young Guen Kwon, Young Myeong Kim

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Prostaglandin E2 (PGE2), a major product of cyclooxygenase, has been implicated in modulating angiogenesis, vascular function, and inflammatory processes, but the underlying mechanism is not clearly elucidated. We here investigated the molecular mechanism by which PGE2 regulates angiogenesis. Treatment of human umbilical vein endothelial cells (HUVEC) with PGE2 increased angiogenesis. PGE 2 increased phosphorylation of Akt and endothelial nitric oxide synthase (eNOS), eNOS activity, and nitric oxide (NO) production by the activation of cAMP-dependent protein kinase (PKA) and phosphatidylinositol 3-kinase (PI3K). Dibutyryl cAMP (DB-cAMP) mimicked the role of PGE2 in angiogenesis and the signaling pathway, suggesting that cAMP is a down-stream mediator of PGE2. Furthermore, PGE2 increased endothelial cell sprouting from normal murine aortic segments, but not from eNOS-deficient ones, on Matrigel. The angiogenic effects of PGE2 were inhibited by the inhibitors of PKA, PI3K, eNOS, and soluble guanylate cyclase, but not by phospholipase C inhibitor. These results clearly show that PGE2 increased angiogenesis by activating the NO/cGMP signaling pathway through PKA/PI3K/Akt-dependent increase in eNOS activity.

Original languageEnglish (US)
Pages (from-to)588-600
Number of pages13
JournalExperimental and Molecular Medicine
Volume37
Issue number6
DOIs
StatePublished - Dec 31 2005

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Endothelial cells
Human Umbilical Vein Endothelial Cells
Dinoprostone
Nitric Oxide
Nitric Oxide Synthase Type III
Phosphatidylinositol 3-Kinase
Phosphorylation
Guanylate Cyclase
Type C Phospholipases
Protein Kinase Inhibitors
Prostaglandin-Endoperoxide Synthases
Cyclic AMP-Dependent Protein Kinases
Prostaglandins E
Protein Kinases
Blood Vessels
Endothelial Cells
Chemical activation
Proteins

Keywords

  • Cyclic AMP
  • Endothelial cells
  • Endothelial nitric oxide synthase
  • Prostaglandin E

Cite this

Prostaglandin E2 stimulates angiogenesis by activating the nitric oxide/cGMP pathway in human umbilical vein endothelial cells. / Namkoong, Seung; Lee, Seon Jin; Kim, Chun Ki; Kim, Young Mi; Chung, Hun Taeg; Lee, Hansoo; Han, Jeong A.; Ha, Kwon Soo; Kwon, Young Guen; Kim, Young Myeong.

In: Experimental and Molecular Medicine, Vol. 37, No. 6, 31.12.2005, p. 588-600.

Research output: Contribution to journalArticle

Namkoong, S, Lee, SJ, Kim, CK, Kim, YM, Chung, HT, Lee, H, Han, JA, Ha, KS, Kwon, YG & Kim, YM 2005, 'Prostaglandin E2 stimulates angiogenesis by activating the nitric oxide/cGMP pathway in human umbilical vein endothelial cells', Experimental and Molecular Medicine, vol. 37, no. 6, pp. 588-600. https://doi.org/10.1038/emm.2005.72
Namkoong, Seung ; Lee, Seon Jin ; Kim, Chun Ki ; Kim, Young Mi ; Chung, Hun Taeg ; Lee, Hansoo ; Han, Jeong A. ; Ha, Kwon Soo ; Kwon, Young Guen ; Kim, Young Myeong. / Prostaglandin E2 stimulates angiogenesis by activating the nitric oxide/cGMP pathway in human umbilical vein endothelial cells. In: Experimental and Molecular Medicine. 2005 ; Vol. 37, No. 6. pp. 588-600.
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