TY - JOUR
T1 - Prostaglandin catabolism in fetal and maternal tissues - A study of 15-hydroxyprostaglandin dehydrogenase and Δ13 reductase with specific assay methods
AU - Tsai, Michael Y
AU - Einzig, S.
N1 - Funding Information:
expert technical assistanceT. his study was supportedb y a grant from the American Heart Association, Minnesota Affiliate.
PY - 1989/10
Y1 - 1989/10
N2 - Recent studies have demonstrated that extraductal tissues such as lung are important sources of prostaglandin E2 which maintains the patency of ductus arteriosus in fetuses and prematurely-born infants. Also, organs such as lung are known to be active in the catabolism of PGE2. Earlier studies of enzymes involved in the catabolism of PGE2 such as 15-hydroxyprostaglandin dehydrogenase (15-PGDH) and Δ13 reductase all used non-specific methods. In the present report, we studied 15-PGDH in fetal and maternal rat lung, kidney, and fetal lamb lung, kidney and ductus arteriosus with the use of a specific substrate (15-S)-[153H-PGE2]. In addition, we measured the activity of Δ13 reductase in these tissues by measuring the conversion of [1-14C]-15-keto PGE2 to [1-14C]-15-keto-13,14-dihydro PGE2. The results from these studies demonstrated that in fetal rat lung and kidney, 15-PGDH activities increased rapidly while Δ13 reductase remained unchanged during late gestation. Ductus arteriosus possessed little 15-PGDH activities. These results strongly suggest that extraductal regulation of PGE2 metabolism is important in determining ductal caliber in fetuses and prematurely delivered neonates.
AB - Recent studies have demonstrated that extraductal tissues such as lung are important sources of prostaglandin E2 which maintains the patency of ductus arteriosus in fetuses and prematurely-born infants. Also, organs such as lung are known to be active in the catabolism of PGE2. Earlier studies of enzymes involved in the catabolism of PGE2 such as 15-hydroxyprostaglandin dehydrogenase (15-PGDH) and Δ13 reductase all used non-specific methods. In the present report, we studied 15-PGDH in fetal and maternal rat lung, kidney, and fetal lamb lung, kidney and ductus arteriosus with the use of a specific substrate (15-S)-[153H-PGE2]. In addition, we measured the activity of Δ13 reductase in these tissues by measuring the conversion of [1-14C]-15-keto PGE2 to [1-14C]-15-keto-13,14-dihydro PGE2. The results from these studies demonstrated that in fetal rat lung and kidney, 15-PGDH activities increased rapidly while Δ13 reductase remained unchanged during late gestation. Ductus arteriosus possessed little 15-PGDH activities. These results strongly suggest that extraductal regulation of PGE2 metabolism is important in determining ductal caliber in fetuses and prematurely delivered neonates.
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U2 - 10.1016/0952-3278(89)90143-9
DO - 10.1016/0952-3278(89)90143-9
M3 - Article
C2 - 2558389
AN - SCOPUS:0024461158
SN - 0952-3278
VL - 38
SP - 25
EP - 30
JO - Prostaglandins, Leukotrienes and Essential Fatty Acids
JF - Prostaglandins, Leukotrienes and Essential Fatty Acids
IS - 1
ER -