1. 1. The properties of a soluble ribonuclease from Aedes aegypti larvae have been compared with ribonuclease activity in adult female tissue. 2. 2. In larval extracts ribonuclease activity was maximal at 40-45°C whereas activity in tissue from adult females was highest at 50°C. 3. 3. Ribonuclease activity that was recovered in a 20-60% ammonium sulfate precipitate was further purified by batch elution from DEAE-Sephacel and from carboxymethylcellulose. 4. 4. Ribonuclease activity in the partially purified fraction was sensitive to EDTA, stimulated by magnesium, had a pH optimum at 9.0 and a Mr of 45,000. 5. 5. Agarose gels containing yeast RNA substrate were used to monitor partial purification of the larval ribonuclease.
|Original language||English (US)|
|Number of pages||7|
|Journal||Comparative Biochemistry and Physiology -- Part B: Biochemistry and|
|State||Published - 1987|