Properties of a ribonuclease from Aedes aegypti larvae

Mary Ann Fritz, Ann Marie Fallon

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

1. 1. The properties of a soluble ribonuclease from Aedes aegypti larvae have been compared with ribonuclease activity in adult female tissue. 2. 2. In larval extracts ribonuclease activity was maximal at 40-45°C whereas activity in tissue from adult females was highest at 50°C. 3. 3. Ribonuclease activity that was recovered in a 20-60% ammonium sulfate precipitate was further purified by batch elution from DEAE-Sephacel and from carboxymethylcellulose. 4. 4. Ribonuclease activity in the partially purified fraction was sensitive to EDTA, stimulated by magnesium, had a pH optimum at 9.0 and a Mr of 45,000. 5. 5. Agarose gels containing yeast RNA substrate were used to monitor partial purification of the larval ribonuclease.

Original languageEnglish (US)
Pages (from-to)595-601
Number of pages7
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume88
Issue number2
DOIs
StatePublished - 1987

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