TY - JOUR
T1 - Propagation and maintenance of undifferentiated human embryonic stem cells
AU - Bodnar, Megan S.
AU - Meneses, Juanito J.
AU - Rodriguez, Ryan T.
AU - Firpo, Meri T.
PY - 2004/6/1
Y1 - 2004/6/1
N2 - Human embryonic stem (hES) cells, like other stem cells, have the capacity to self-renew without differentiation. Although hES cells can be differentiated to many different tissue types in vitro, clinical uses have not yet been realized from the study of hES cells. Anticipation that these cells would be immediately useful for creating models of human disease has not yet been fulfilled. However, because of their self-renewing and pluripotential nature, hES cells indeed hold unique promise for many areas of research and medicine. A major problem complicating developments in hES cell research is the difficulty of propagating and maintaining these cells in vitro without differentiation. This review addresses this problem and potential solutions in detail. In addition, the current state of research regarding the growth and maintenance of hES cells is summarized, along with basic protocols utilized by our laboratory for the successful propagation, characterization, and investigation of hES cells.
AB - Human embryonic stem (hES) cells, like other stem cells, have the capacity to self-renew without differentiation. Although hES cells can be differentiated to many different tissue types in vitro, clinical uses have not yet been realized from the study of hES cells. Anticipation that these cells would be immediately useful for creating models of human disease has not yet been fulfilled. However, because of their self-renewing and pluripotential nature, hES cells indeed hold unique promise for many areas of research and medicine. A major problem complicating developments in hES cell research is the difficulty of propagating and maintaining these cells in vitro without differentiation. This review addresses this problem and potential solutions in detail. In addition, the current state of research regarding the growth and maintenance of hES cells is summarized, along with basic protocols utilized by our laboratory for the successful propagation, characterization, and investigation of hES cells.
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U2 - 10.1089/154732804323099172
DO - 10.1089/154732804323099172
M3 - Review article
C2 - 15186720
AN - SCOPUS:2942527400
VL - 13
SP - 243
EP - 253
JO - Stem Cells and Development
JF - Stem Cells and Development
SN - 1547-3287
IS - 3
ER -