Proliferative effect of interleukin-3 on normal and leukemic human B cell precursors

B. Wormann, T. G. Gesner, R. A. Mufson, Tucker W LeBien

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

We have examined the influence of recombinant, human IL-3 on normal and leukemic B cell precursors. CD10/CALLA+ leukemic B cell precursors, the pre-B cell line BLIN-1, and surface IgM- B cell precursors isolated from fetal bone marrow all responded to IL-3. This IL-3 response was dose-dependent and could be abrogated by a rabbit anti-IL-3 antiserum. Binding studies using radiolabeled Il-3 revealed the presence of approximately 200 high affinity IL-3 receptors on the BLIN-1 cell line, with a K(D) of 150 pM. We conclude that normal and leukemic human B cell precursors express functional IL-3 receptors, extending the biologic range of this hematopoietin to the lymphoid lineage.

Original languageEnglish (US)
Pages (from-to)399-404
Number of pages6
JournalLeukemia
Volume3
Issue number6
StatePublished - 1989

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