Progesterone supplementation to lactating dairy cows without a corpus luteum at initiation of the Ovsynch protocol

R. S. Bisinotto, L. O. Castro, M. B. Pansani, C. D. Narciso, N. Martinez, L. D.P. Sinedino, T. L.C. Pinto, N. S. Van de Burgwal, H. M. Bosman, R. S. Surjus, W. W. Thatcher, J. E.P. Santos

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48 Scopus citations


The objectives were to determine the effect of progesterone supplementation on fertility responses in lactating dairy cows without corpora lutea (CL) at initiation of the timed artificial insemination (AI) program. Holstein cows from 5 commercial dairy farms were subjected to the Ovsynch-56 protocol (d -10 GnRH, d -3 PGF, d -0.7 GnRH, d 0 AI). Ovaries were scanned by ultrasonography on d -10. Within farm, cows without CL were blocked by pen and assigned randomly to remain as nonsupplemented controls (CON; n=652) or to receive 2 controlled internal drug-release (CIDR) inserts containing 1.38g of progesterone each from d -10 to -3 (2CIDR; n=642). Cows with CL were randomly selected within pen and used as positive controls as cows in diestrus at the initiation of the Ovsynch protocol (DIEST; n=640). Signs of estrus were detected beginning on d -9 based on removal of tail chalk, and cows in estrus received AI on the same day. Blood samples from subsets of cows on d -10, -9, -7, -5, -3, and 0 (n=109) and on d 6, 13, and 19 (n=156) were analyzed for progesterone concentrations. Pregnancy was diagnosed on d 32 and 60 after AI. The average progesterone concentration during the timed AI program was lowest for CON, intermediate for 2CIDR, and highest for DIEST (0.92, 2.77, and 4.93 ng/mL, respectively). The proportions of cows that ovulated in response to the first GnRH (63.6, 61.1, and 47.2%, respectively) and that had a new CL on d -3 at PGF injection (72.4, 67.9, and 47.4%, respectively) were greater for CON and 2CIDR compared with DIEST, respectively. The diameter of the ovulatory follicle and the proportion of cows that ovulated in response to the second GnRH did not differ among treatments. A greater proportion of CON and 2CIDR cows were detected in estrus at AI compared with DIEST cows (35.8, 39.6, and 30.6%, respectively). Pregnancy per AI was less for CON compared with 2CIDR and DIEST on d 32 (31.3, 42.2, and 38.4%, respectively) and d 60 after AI (28.9, 37.2, and 33.9%, respectively), indicating that progesterone supplementation reestablished fertility in cows lacking a CL similar to that of cows in diestrus at the initiation of the timed AI program. Treatment did not affect pregnancy loss between d 32 and 60 of gestation. Pregnancy from a subset of cows with plasma progesterone concentrations indicated that a minimum concentration of 2.0 ng/mL was needed to optimize fertility. A single ultrasound examination effectively identified a low-fertility cohort of cows based on the absence of CL at the first GnRH injection of the Ovsynch protocol. Supplementation with 2 CIDR inserts increased progesterone in plasma by an additional 1.85 ng/mL compared with CON, resulting in concentrations of 2.77 ng/mL during development of the ovulatory follicle, which restored fertility in dairy cows lacking CL to a level similar to that of cows in diestrus.

Original languageEnglish (US)
Pages (from-to)2515-2528
Number of pages14
JournalJournal of Dairy Science
Issue number4
StatePublished - Apr 1 2015
Externally publishedYes

Bibliographical note

Funding Information:
The authors thank the owners and staff of Alliance Dairies (Trenton, FL), River Ranch Farms (Hanford, CA), John de Jong Dairy (Hanford, CA), and High Roller Dairy (Hanford, CA) for the use of their cows and facilities and assistance during experimental procedures. The assistance of Eduardo S. Ribeiro, Fábio S. Lima, Gabriel C. Gomes, Rodolfo D. Medina, and Thiago F. Fabris from the University of Florida (Gainesville) is appreciated. Financial support for this project was provided by a grant from the Southeast Milk Inc. Checkoff Program (Belleview, FL) .

Publisher Copyright:
© 2015 American Dairy Science Association.


  • Anovular cow
  • Corpus luteum
  • Progesterone
  • Reproduction


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