Profiling DNA methylation by bisulfite genomic sequencing: problems and solutions.

Liang Liu, Rebecca C. Wylie, Nathaniel J. Hansen, Lucy G. Andrews, Trygve O. Tollefsbol

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The surge of interest in DNA methylation during the last two decades has triggered an urgent need for an effective method to detect the methylation status of the cytosines in the genome. Bisulfite genomic sequencing is the most attractive choice so far for many laboratories. Various protocols have been established, but difficulties are often encountered, particularly by individuals who have limited experience in this field. This analysis presents a simple protocol that has consistently worked well in our laboratory. Discussions of potential technical problems and corresponding solutions are also included to facilitate the reproducibility of this protocol.

Original languageEnglish (US)
Pages (from-to)169-179
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume287
DOIs
StatePublished - 2004
Externally publishedYes

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