Productive life cycle of adeno-associated virus serotype 2 in the complete absence of a conventional polyadenylation signal

Lina Wang, Zifei Yin, Yuan Wang, Yuan Lu, Daniel Zhang, Arun Srivastava, Changquan Ling, George V. Aslanidi, Chen Ling

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We showed that WT adeno-associated virus serotype 2 (AAV2) genome devoid of a conventional polyadenylation [poly(A)] signal underwent complete genome replication, encapsidation and progeny virion production in the presence of adenovirus. The infectivity of the progeny virion was also retained. Using recombinant AAV2 vectors devoid of a human growth hormone poly(A) signal, we also demonstrated that a subset of mRNA transcripts contained the inverted terminal repeat (ITR) sequence at the 39 end, which we designated ITR in RNA (ITRR). Furthermore, AAV replication (Rep) proteins were able to interact with the ITRR. Taken together, our studies suggest a new function of the AAV2 ITR as an RNA element to mediate transgene expression from poly(A)-deleted mRNA.

Original languageEnglish (US)
Pages (from-to)2780-2787
Number of pages8
JournalJournal of General Virology
Volume96
Issue number9
DOIs
StatePublished - Sep 1 2015
Externally publishedYes

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