Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7

Odayme Quesada; Brittney Gurda; Lakshmanan Govindasamy; Robert McKenna; Erik Kohlbrenner; George Aslanidi; Sergei Zolotukhin; Nicholas Muzyczka; Mavis Agbandje-Mckenna

doi:10.1107/S1744309107060289

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7

Odayme Quesada, Brittney Gurda, Lakshmanan Govindasamy, Robert McKenna, Erik Kohlbrenner, George Aslanidi, Sergei Zolotukhin, Nicholas Muzyczka, Mavis Agbandje-Mckenna

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to ∼3.0 Å resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 Å in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R merge of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.

Original language	English (US)
Pages (from-to)	1073-1076
Number of pages	4
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	63
Issue number	12
DOIs	https://doi.org/10.1107/S1744309107060289
State	Published - Nov 30 2007
Externally published	Yes

Keywords

Adeno-associated virus serotype 7

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10.1107/S1744309107060289

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Quesada, O., Gurda, B., Govindasamy, L., McKenna, R., Kohlbrenner, E., Aslanidi, G., Zolotukhin, S., Muzyczka, N., & Agbandje-Mckenna, M. (2007). Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 63(12), 1073-1076. https://doi.org/10.1107/S1744309107060289

Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7. / Quesada, Odayme; Gurda, Brittney; Govindasamy, Lakshmanan et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 63, No. 12, 30.11.2007, p. 1073-1076.

Research output: Contribution to journal › Article › peer-review

Quesada, O, Gurda, B, Govindasamy, L, McKenna, R, Kohlbrenner, E, Aslanidi, G, Zolotukhin, S, Muzyczka, N & Agbandje-Mckenna, M 2007, 'Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 63, no. 12, pp. 1073-1076. https://doi.org/10.1107/S1744309107060289

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abstract = "Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to ∼3.0 {\AA} resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 {\AA} in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R merge of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.",

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AU - Quesada, Odayme

AU - Gurda, Brittney

AU - Govindasamy, Lakshmanan

AU - McKenna, Robert

AU - Kohlbrenner, Erik

AU - Aslanidi, George

AU - Zolotukhin, Sergei

AU - Muzyczka, Nicholas

AU - Agbandje-Mckenna, Mavis

PY - 2007/11/30

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N2 - Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to ∼3.0 Å resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 Å in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R merge of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.

AB - Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to ∼3.0 Å resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 Å in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R merge of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.

KW - Adeno-associated virus serotype 7

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Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7

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