TY - JOUR
T1 - Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 9
AU - Mitchell, Matthew
AU - Nam, Hyun Joo
AU - Carter, Adam
AU - McCall, Angela
AU - Rence, Chelsea
AU - Bennett, Antonette
AU - Gurda, Brittney
AU - McKenna, Robert
AU - Porter, Mark
AU - Sakai, Yoshihisa
AU - Byrne, Barry J.
AU - Muzyczka, Nicholas
AU - Aslanidi, George
AU - Zolotukhin, Sergei
AU - Agbandje-Mckenna, Mavis
PY - 2009
Y1 - 2009
N2 - Adeno-associated virus (AAV) serotype 9, which is under development for gene-delivery applications, shows significantly enhanced capsid-associated transduction efficiency in muscle compared with other AAV serotypes. With the aim of characterizing the structural determinants of this property, the purification, crystallization and preliminary X-ray crystallographic analyses of the AAV9 viral capsid are reported. The crystals diffracted X-rays to 2.8 Å resolution using synchrotron radiation and belonged to the trigonal space group P32, with unit-cell parameters a = b = 251.0, c = 640.0 Å. There are three complete viral capsids in the crystal unit cell. The orientation and position of the asymmetric unit capsid have been determined by molecular-replacement methods and structure determination is in progress.
AB - Adeno-associated virus (AAV) serotype 9, which is under development for gene-delivery applications, shows significantly enhanced capsid-associated transduction efficiency in muscle compared with other AAV serotypes. With the aim of characterizing the structural determinants of this property, the purification, crystallization and preliminary X-ray crystallographic analyses of the AAV9 viral capsid are reported. The crystals diffracted X-rays to 2.8 Å resolution using synchrotron radiation and belonged to the trigonal space group P32, with unit-cell parameters a = b = 251.0, c = 640.0 Å. There are three complete viral capsids in the crystal unit cell. The orientation and position of the asymmetric unit capsid have been determined by molecular-replacement methods and structure determination is in progress.
KW - Adeno-associate virus serotype 9
KW - Viruses
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U2 - 10.1107/S1744309109021460
DO - 10.1107/S1744309109021460
M3 - Article
C2 - 19574648
AN - SCOPUS:68149134777
SN - 1744-3091
VL - 65
SP - 715
EP - 718
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 7
ER -