Production of the gaseous signal molecule hydrogen sulfide in mouse tissues

David R. Linden, Lei Sha, Amelia Mazzone, Gary J. Stoltz, Cheryl E. Bernard, Julie K. Furne, Michael D. Levitt, Gianrico Farrugia, Joseph H. Szurszewski

Research output: Contribution to journalArticlepeer-review

139 Scopus citations


The gaseous molecule hydrogen sulfide (H2S) has been proposed as an endogenous signal molecule and neuromodulator in mammals. Using a newly developed method, we report here for the first time the ability of intact and living brain and colonic tissue in the mouse to generate and release H 2S. This production occurs through the activity of two enzymes, cystathionine-γ-lyase and cystathionine-β-synthase. The quantitative expression of messenger RNA and protein localization for both enzymes are described in the liver, brain, and colon. Expression levels of the enzymes vary between tissues and are differentially distributed. The observation that, tissues that respond to exogenously applied H2S can endogenously generate the gas, strongly supports its role as an endogenous signal molecule.

Original languageEnglish (US)
Pages (from-to)1577-1585
Number of pages9
JournalJournal of Neurochemistry
Issue number4
StatePublished - Aug 2008
Externally publishedYes


  • Aseptic dissection
  • Cysteine catabolism
  • Gastrointestinal
  • Immunohistochemistry
  • Trans-sulfuration


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