Production of engineered minichromosome vectors via the introduction of telomere sequences

Nathaniel Graham, Nathan Swyers, Jon Cody, Morgan McCaw, Changzeng Zhao, James A. Birchler

Research output: Chapter in Book/Report/Conference proceedingChapter


Artificial minichromosomes are non-integrating vectors capable of stably maintaining transgenes outside of the main chromosome set. The production of minichromosomes relies on telomere-mediated chromosomal truncation, which involves introducing transgenes and telomere sequences concurrently to the cell to truncate an endogenous chromosomal target. Two methods can be utilized; either the telomere sequences can be incorporated into a binary vector for transformation with Agrobacterium tumefaciens, or the telomere sequences can be co-introduced with transgenes during particle bombardment. In this protocol, the methods required to isolate and introduce telomere sequences are presented. Following the methods presented, standard transformation procedures can be followed to produce minichromosome containing plants.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
This work was supported by NSF grant IOS-1339198 from the Plant Genome Program.

Publisher Copyright:
© Springer Science+Business Media New York 2016.


  • Agrobacterium tumefaciens transformation
  • Artificial chromosome
  • Biolistic bombardment
  • Chromosomal truncation
  • In-gel ligation
  • Minichromosome
  • Plant transformation
  • Telomere


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