Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway

Sunyoung Kang, Bo Min Kim, Kyung Taek Heo, Jae Hyuk Jang, Won Gon Kim, Young Soo Hong

Research output: Contribution to journalArticle

Abstract

A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.

Original languageEnglish (US)
Pages (from-to)2104-2111
Number of pages8
JournalJournal of Microbiology and Biotechnology
Volume27
Issue number12
DOIs
StatePublished - Dec 1 2017

Fingerprint

Quorum Sensing
Biosynthetic Pathways
Escherichia coli
homoserine lactone
Agrobacterium tumefaciens
Methionine
Genes
Tyrosine
Carbon

Keywords

  • Artificial biosynthesis
  • Caffeoyl-HSL
  • Feruloyl-HSL
  • Phenylacetyl homoserine lactone

Cite this

Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway. / Kang, Sunyoung; Kim, Bo Min; Heo, Kyung Taek; Jang, Jae Hyuk; Kim, Won Gon; Hong, Young Soo.

In: Journal of Microbiology and Biotechnology, Vol. 27, No. 12, 01.12.2017, p. 2104-2111.

Research output: Contribution to journalArticle

Kang, Sunyoung ; Kim, Bo Min ; Heo, Kyung Taek ; Jang, Jae Hyuk ; Kim, Won Gon ; Hong, Young Soo. / Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway. In: Journal of Microbiology and Biotechnology. 2017 ; Vol. 27, No. 12. pp. 2104-2111.
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