Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway

Sun Young Kang; Bo Min Kim; Kyung Taek Heo; Jae Hyuk Jang; Won Gon Kim; Young Soo Hong

doi:10.4014/jmb.1705.05033

Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway

Sun Young Kang, Bo Min Kim, Kyung Taek Heo, Jae Hyuk Jang, Won Gon Kim, Young Soo Hong

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.

Original language	English (US)
Pages (from-to)	2104-2111
Number of pages	8
Journal	Journal of Microbiology and Biotechnology
Volume	27
Issue number	12
DOIs	https://doi.org/10.4014/jmb.1705.05033
State	Published - Dec 2017
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, Grant No. PJ0110842017), Rural Development Administration, Republic of Korea.

Publisher Copyright:
© 2017 by The Korean Society for Microbiology and Biotechnology.

Keywords

Artificial biosynthesis
Caffeoyl-HSL
Feruloyl-HSL
Phenylacetyl homoserine lactone

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10.4014/jmb.1705.05033

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title = "Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway",

abstract = "A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.",

keywords = "Artificial biosynthesis, Caffeoyl-HSL, Feruloyl-HSL, Phenylacetyl homoserine lactone",

author = "Kang, {Sun Young} and Kim, {Bo Min} and Heo, {Kyung Taek} and Jang, {Jae Hyuk} and Kim, {Won Gon} and Hong, {Young Soo}",

note = "Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, Grant No. PJ0110842017), Rural Development Administration, Republic of Korea. Publisher Copyright: {\textcopyright} 2017 by The Korean Society for Microbiology and Biotechnology.",

year = "2017",

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doi = "10.4014/jmb.1705.05033",

language = "English (US)",

volume = "27",

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T1 - Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway

AU - Kang, Sun Young

AU - Kim, Bo Min

AU - Heo, Kyung Taek

AU - Jang, Jae Hyuk

AU - Kim, Won Gon

AU - Hong, Young Soo

N1 - Funding Information: This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, Grant No. PJ0110842017), Rural Development Administration, Republic of Korea. Publisher Copyright: © 2017 by The Korean Society for Microbiology and Biotechnology.

PY - 2017/12

Y1 - 2017/12

N2 - A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.

AB - A new series comprising phenylacetyl-homoserine lactones (HSLs), caffeoyl-HSL and feruloyl-HSL, was biologically synthesized using an artificial de novo biosynthetic pathway. We developed an Escherichia coli system containing artificial biosynthetic pathways that yield phenylacetyl-HSLs from simple carbon sources. These artificial biosynthetic pathways contained the LuxI-type synthase gene (rpaI) in addition to caffeoyl-CoA and feruloyl-CoA biosynthetic genes, respectively. Finally, the yields for caffeoyl-HSL and feruloyl-HSL were 97.1 ± 10.3 and 65.2 ± 5.7 mg/l, respectively, by tyrosine-overproducing E. coli with a L-methionine feeding strategy. In a quorum sensing (QS) competition assay, feruloyl-HSL and p-coumaroyl-HSL antagonized the QS receptor TraR in Agrobacterium tumefaciens NT1, whereas caffeoyl-HSL did not.

KW - Artificial biosynthesis

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KW - Feruloyl-HSL

KW - Phenylacetyl homoserine lactone

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Production of bacterial quorum sensing antagonists, caffeoyl- and Feruloyl-HSL, by an artificial biosynthetic pathway

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