Production of 2,3-butanediol from xylose by engineered Saccharomyces cerevisiae

Soo Jung Kim, Seung Oh Seo, Yong Cheol Park, Yong Su Jin, Jin Ho Seo

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


2,3-Butanediol (2,3-BD) production from xylose that is abundant in lignocellulosic hydrolyzate would make the production of 2,3-BD more sustainable and economical. Saccharomyces cerevisiae can produce only trace amounts of 2,3-BD, but also cannot ferment xylose. Therefore, it is necessary to introduce both 2,3-BD production and xylose assimilation pathways into S. cerevisiae for producing 2,3-BD from xylose. A pyruvate decarboxylase (Pdc)-deficient mutant (SOS4) was used as a host in order to increase carbon flux toward 2,3-BD instead of ethanol. The XYL1, XYL2, and XYL3 genes coding for xylose assimilating enzymes derived from Scheffersomyces stipitis were introduced into the SOS4 strain to enable xylose utilization. Additionally, the alsS and alsD genes from Bacillus subtilis and endogenous BDH1 gene were overexpressed to increase 2,3-BD production from xylose. As a result, the resulting strain (BD4X) produced 20.7 g/L of 2,3-BD from xylose with a yield of 0.27 g 2,3-BD/g xylose. The titer of 2,3-BD from xylose increased up to 43.6 g/L under a fed-batch fermentation. The BD4X strain produced (R, R)-2,3-BD dominantly (>97% of the total 2,3-BD) with trace amounts of meso-2,3-BD. These results suggest that S. cerevisiae might be a promising host for producing 2,3-BD from lignocellulosic biomass for industrial applications.

Original languageEnglish (US)
Pages (from-to)376-382
Number of pages7
JournalJournal of Biotechnology
StatePublished - Dec 20 2014

Bibliographical note

Funding Information:
This research was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant ( 2010-0029799 ) funded by the Ministry of Education, Science and Technology and Korea Research Council of Fundamental Science & Technology (KRCF) grant.

Publisher Copyright:
© 2014 Elsevier B.V.


  • 2,3-Butanediol (2,3-BD)
  • Pyruvate decarboxylase-deficient mutant
  • Saccharomyces cerevisiae
  • Xylose


Dive into the research topics of 'Production of 2,3-butanediol from xylose by engineered Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this