Production of 2,3-butanediol by engineered saccharomyces cerevisiae

Soo Jung Kim, Seung Oh Seo, Yong Su Jin, Jin Ho Seo

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

In order to produce 2,3-butanediol (2,3-BD) with a high titer, it is necessary to engineer Saccharomyces cerevisiae by deleting the competing pathway and overexpressing the 2,3-BD biosynthetic pathway. A pyruvate decarboxylase (Pdc)-deficient mutant was constructed and evolved for rapid glucose consumption without ethanol production. Genome re-sequencing of the evolved strain (SOS4) revealed a point mutation (A81P) in MTH1 coding for a transcriptional regulator involved in glucose sensing, unlike the previously reported Pdc-deficient mutant which had internal deletion in MTH1. When alsS and alsD genes from Bacillus subtilis, and endogenous BDH1 gene were overexpressed in SOS4, the resulting strain (BD4) not only produced 2,3-BD efficiently, but also consumed glucose faster than the parental strain. In fed-batch fermentation with optimum aeration, 2,3-BD concentration increased up to 96.2. g/L. These results suggest that S. cerevisiae might be a promising host for producing 2,3-BD for industrial applications.

Original languageEnglish (US)
Pages (from-to)274-281
Number of pages8
JournalBioresource Technology
Volume146
DOIs
StatePublished - Oct 2013

Bibliographical note

Funding Information:
This research was supported by the Advanced Biomass R&D Center (ABC) of Korea Grant ( 2010-0029799 ) funded by the Ministry of Science, ICT & Future Planning and by Korea Research Council of Fundamental Science & Technology (KRCF) Grant.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • 2,3-Butanediol (2,3-BD)
  • MTH1
  • Pyruvate decarboxylase-deficient mutant
  • Saccharomyces cerevisiae

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