Production and Purification of Functional Cryptosporidium Glycoproteins by Heterologous Expression in Toxoplasma gondii

Iwona Driskell, Roberta M. O’Connor

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Development of an effective vaccine against cryptosporidiosis is a medical and veterinary priority. However, many putative Cryptosporidium vaccine candidates such as surface and apical complex antigens are posttranslationally modified with O- and N-linked glycans. This presents a significant challenge to understanding the functions of these antigens and the immune responses to them. Isolation of large amounts of native antigen from Cryptosporidium oocysts is expensive and is only feasible for C. parvum antigens. Here, we describe a method of producing recombinant, functional Cryptosporidium glycoprotein antigens in Toxoplasma gondii. These functional recombinant proteins can be used to investigate the role of glycotopes in Cryptosporidium immune responses and parasite–host cell interactions.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages87-102
Number of pages16
DOIs
StatePublished - 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2052
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2020, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Affinity purification
  • Cryptosporidium
  • Glycoproteins
  • Glycotopes
  • Surface antigens
  • Toxoplasma

PubMed: MeSH publication types

  • Journal Article

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