Producing Engraftable Skeletal Myogenic Progenitors from Pluripotent Stem Cells via Teratoma Formation

Ning Xie, Sunny S.K. Chan

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Generating engraftable skeletal muscle progenitor cells is a promising cell therapy approach to treating degenerating muscle diseases. Pluripotent stem cell (PSC) is an ideal cell source for cell therapy because of its unlimited proliferative capability and potential to differentiate into multiple lineages. Approaches such as ectopic overexpression of myogenic transcription factors and growth factors–directed monolayer differentiation, while able to differentiate PSCs into the skeletal myogenic lineage in vitro, are limited in producing muscle cells that reliably engraft upon transplantation. Here we present a novel method to differentiate mouse PSCs into skeletal myogenic progenitors without genetic modification or monolayer culture. We make use of forming a teratoma, in which skeletal myogenic progenitors can be routinely obtained. We first inject mouse PSCs into the limb muscle of an immuno-compromised mouse. Within 3–4 weeks, α7-integrin+ VCAM-1+ skeletal myogenic progenitors are purified by fluorescent-activated cell sorting. We further transplant these teratoma-derived skeletal myogenic progenitors into dystrophin-deficient mice to assess engraftment efficiency. This teratoma formation strategy is capable of generating skeletal myogenic progenitors with high regenerative potency from PSCs without genetic modifications or growth factors supplementation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages175-189
Number of pages15
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2640
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2023, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Cell therapy
  • Cell transplantation
  • Muscle stem cell
  • Pluripotent stem cell
  • Skeletal myogenic progenitor
  • Teratoma
  • Satellite Cells, Skeletal Muscle/metabolism
  • Intercellular Signaling Peptides and Proteins
  • Muscle, Skeletal/metabolism
  • Muscle Development
  • Animals
  • Pluripotent Stem Cells
  • Mice
  • Cell Differentiation
  • Teratoma/etiology
  • Muscle Fibers, Skeletal/metabolism

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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