Abstract
Generating engraftable skeletal muscle progenitor cells is a promising cell therapy approach to treating degenerating muscle diseases. Pluripotent stem cell (PSC) is an ideal cell source for cell therapy because of its unlimited proliferative capability and potential to differentiate into multiple lineages. Approaches such as ectopic overexpression of myogenic transcription factors and growth factors–directed monolayer differentiation, while able to differentiate PSCs into the skeletal myogenic lineage in vitro, are limited in producing muscle cells that reliably engraft upon transplantation. Here we present a novel method to differentiate mouse PSCs into skeletal myogenic progenitors without genetic modification or monolayer culture. We make use of forming a teratoma, in which skeletal myogenic progenitors can be routinely obtained. We first inject mouse PSCs into the limb muscle of an immuno-compromised mouse. Within 3–4 weeks, α7-integrin+ VCAM-1+ skeletal myogenic progenitors are purified by fluorescent-activated cell sorting. We further transplant these teratoma-derived skeletal myogenic progenitors into dystrophin-deficient mice to assess engraftment efficiency. This teratoma formation strategy is capable of generating skeletal myogenic progenitors with high regenerative potency from PSCs without genetic modifications or growth factors supplementation.
Original language | English (US) |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 175-189 |
Number of pages | 15 |
DOIs | |
State | Published - 2023 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2640 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© 2023, Springer Science+Business Media, LLC, part of Springer Nature.
Keywords
- Cell therapy
- Cell transplantation
- Muscle stem cell
- Pluripotent stem cell
- Skeletal myogenic progenitor
- Teratoma
- Satellite Cells, Skeletal Muscle/metabolism
- Intercellular Signaling Peptides and Proteins
- Muscle, Skeletal/metabolism
- Muscle Development
- Animals
- Pluripotent Stem Cells
- Mice
- Cell Differentiation
- Teratoma/etiology
- Muscle Fibers, Skeletal/metabolism
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't