Probing subtle fluorescence dynamics in cellular proteins by streak camera based fluorescence lifetime imaging microscopy

R. V. Krishnan, Eva Biener, Jian Hua Zhang, Robert Heckel, Brian Herman

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

An overview is given on the possibility to measure fluorescence dynamics of cellular proteins with high spatial/temporal resolution. It is shown that such measurement capability yield useful guidelines in designing proper controls for quantitative live cell imaging such as FRET microscopy and in situ pH imaging.

Original languageEnglish (US)
Pages (from-to)4658-4660
Number of pages3
JournalApplied Physics Letters
Volume83
Issue number22
DOIs
StatePublished - Dec 1 2003

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