Probing biological activities with peptide and peptidomimetic biosensors

Laura J Marholz; Tzu Yi Yang; Laurie L. Parker

doi:10.1002/9781118970195.ch6

Probing biological activities with peptide and peptidomimetic biosensors

Laura J Marholz, Tzu Yi Yang, Laurie L. Parker

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Peptide and peptidomimetic biosensors can be used in combination with a mass spectrometry-based readout to measure or report highly diverse information from biological systems. For example, these biosensors can help us understand reaction mechanisms, assign enzyme substrates, detect ligand interactions, serve as diagnostic tools, and measure enzymatic activity in vitro and in vivo. This chapter highlights and summarizes major recent contributions in this field and includes a detailed protocol as a working example.

Original language	English (US)
Title of host publication	Mass Spectrometry-Based Chemical Proteomics
Publisher	Wiley
Pages	159-185
Number of pages	27
ISBN (Electronic)	9781118970195
ISBN (Print)	9781118969557
DOIs	https://doi.org/10.1002/9781118970195.ch6
State	Published - Jan 1 2019

Bibliographical note

Publisher Copyright:
© 2019 John Wiley & Sons, Inc.

Keywords

Biological activities
Diagnostic applications
Enzymatic reactions
Ligand interactions
Peptide biosensors
Peptide nucleic acids probe
Peptidomimetic biosensors
Rapid fire mass-spectrometry
Screening inhibitors
Substrate specificity

Publisher link

10.1002/9781118970195.ch6

Find It

Find It at U of M Libraries

Cite this

@inbook{854101df0f2c40dbb7a7ec819cf7cb8c,

title = "Probing biological activities with peptide and peptidomimetic biosensors",

abstract = "Peptide and peptidomimetic biosensors can be used in combination with a mass spectrometry-based readout to measure or report highly diverse information from biological systems. For example, these biosensors can help us understand reaction mechanisms, assign enzyme substrates, detect ligand interactions, serve as diagnostic tools, and measure enzymatic activity in vitro and in vivo. This chapter highlights and summarizes major recent contributions in this field and includes a detailed protocol as a working example.",

keywords = "Biological activities, Diagnostic applications, Enzymatic reactions, Ligand interactions, Peptide biosensors, Peptide nucleic acids probe, Peptidomimetic biosensors, Rapid fire mass-spectrometry, Screening inhibitors, Substrate specificity",

author = "Marholz, {Laura J} and Yang, {Tzu Yi} and Parker, {Laurie L.}",

note = "Publisher Copyright: {\textcopyright} 2019 John Wiley & Sons, Inc.",

year = "2019",

month = jan,

day = "1",

doi = "10.1002/9781118970195.ch6",

language = "English (US)",

isbn = "9781118969557",

pages = "159--185",

booktitle = "Mass Spectrometry-Based Chemical Proteomics",

publisher = "Wiley",

}

TY - CHAP

T1 - Probing biological activities with peptide and peptidomimetic biosensors

AU - Marholz, Laura J

AU - Yang, Tzu Yi

AU - Parker, Laurie L.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Peptide and peptidomimetic biosensors can be used in combination with a mass spectrometry-based readout to measure or report highly diverse information from biological systems. For example, these biosensors can help us understand reaction mechanisms, assign enzyme substrates, detect ligand interactions, serve as diagnostic tools, and measure enzymatic activity in vitro and in vivo. This chapter highlights and summarizes major recent contributions in this field and includes a detailed protocol as a working example.

AB - Peptide and peptidomimetic biosensors can be used in combination with a mass spectrometry-based readout to measure or report highly diverse information from biological systems. For example, these biosensors can help us understand reaction mechanisms, assign enzyme substrates, detect ligand interactions, serve as diagnostic tools, and measure enzymatic activity in vitro and in vivo. This chapter highlights and summarizes major recent contributions in this field and includes a detailed protocol as a working example.

KW - Biological activities

KW - Diagnostic applications

KW - Enzymatic reactions

KW - Ligand interactions

KW - Peptide biosensors

KW - Peptide nucleic acids probe

KW - Peptidomimetic biosensors

KW - Rapid fire mass-spectrometry

KW - Screening inhibitors

KW - Substrate specificity

UR - http://www.scopus.com/inward/record.url?scp=85101705041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85101705041&partnerID=8YFLogxK

U2 - 10.1002/9781118970195.ch6

DO - 10.1002/9781118970195.ch6

M3 - Chapter

AN - SCOPUS:85101705041

SN - 9781118969557

SP - 159

EP - 185

BT - Mass Spectrometry-Based Chemical Proteomics

PB - Wiley

ER -

Probing biological activities with peptide and peptidomimetic biosensors

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this