Pro-enkephalin intermediates in bovine brain and adrenal medulla: Characterization of immunoreactive peptides related to BAM-22P and peptide F

Volker Höllt, Ingeborg Haarmann, Cornelia Grimm, Albert Herz, F. Cankat Tulunay, Horace H. Loh

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Using antibodies against the synthetic opioid peptides BAM-22P and peptide F, immunoreactive (ir-) peptides were measured in bovine brain and adrenal medulla. In addition to the high levels in the adrenal medulla, both ir- peptides were measurable in various areas of the brain with highest concentrations in the anterior hypothalamus. Analysis of the ir- components by gel filtration revealed molecular heterogeneity. Besides peptides with the size of BAM-22P or peptide F, various higher molecular weight species were found. These forms were found in the adrenal medulla in much higher concentrations than in the brain indicating a different processing mechanism for proenkephalin. Synthetic BAM-22P injected intracerebroventricularly into mice produces a substantial analgesia (ED50 6.4 nmole) which is almost as high as that of morphine (ED50 2.8 nmole). This finding and the presence of BAM-22P-like compounds in the brain suggests a role of the enkephalinergic system in pain transmission.

Original languageEnglish (US)
Pages (from-to)1883-1886
Number of pages4
JournalLife Sciences
Volume31
Issue number16-17
DOIs
StatePublished - Jan 1 1982

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