Primer pairs, PCR conditions, and peptide nucleic acid clamps affect fungal diversity assessment from plant root tissues

Keyphrases

• Fungal Diversity 100%
• Primer Pairs 100%
• Root Tissue 100%
• PCR Conditions 100%
• Diversity Assessment 100%
• Peptide nucleic Acid Clamp 100%
• Plant DNA 66%
• Microbial Diversity 33%
• Fungal DNA 33%
• DNA Contamination 33%
• Co-amplification 33%
• High-throughput Sequencing 16%
• Fungal Community 16%
• Specific Primers 16%
• Internal Transcribed Spacer 2 (ITS2) 16%
• Systematic Comparison 16%
• Nettle 16%
• Sequencing Depth 16%
• Plant-associated 16%
• Read Depth 16%
• ITS4 16%
• Urtica Dioica 16%
• OTU Richness 16%
• Mycobiota 16%
• ITS2 Region 16%
• Mock Community 16%
• Diversity Recovery 16%
• Mycorrhizal Specificity 16%

Immunology and Microbiology

• Plant Root 100%
• Peptides 100%
• Microbial Diversity 33%
• DNA Contamination 33%
• High Throughput Sequencing 16%
• Urtica Dioica 16%

Agricultural and Biological Sciences

• Nucleic Acid 100%
• Fungal Communities 16%
• Mycobiota 16%
• Urtica Dioica 16%
• High-Throughput Sequencing 16%