Primary Structure of the Ribosomal DNA Intergenic Spacer from the Mosquito, Aedes albopictus

Gerald D. Baldridge, Ann M. Fallon

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

We have determined the primary structure of a 4.7-kb portion of the ribosomal DNA intergenic spacer from cultured cells of the mosquito, Aedes albopictus. Immediately upstream from the 18S rRNA gene was a 753-bp sequence containing two regions similar to known RNA polymerase I promoters, each preceded by potential transcription termination signals. Upstream from this putative promoter region was a 3.15-kb tandem array of 17 direct repeats with a consensus sequence length of 201 bp. The 201-bp repeats contained imperfect antisense duplications of 11-bp core domain regions in the putative RNA polymerase I promoters, and sequences of possible significance in recombination. Farthest upstream of the 18S rRNA gene was an 803-bp region containing two copies each of 34-, 48-, and 64-bp elements separated by apparently unique sequence. This first detailed structural analysis of a ribosomal DNA intergenic spacer from a member of the lower Diptera has revealed features similar to those described for the higher Diptera as well as conserved motifs presumably critical to rRNA transcription.

Original languageEnglish (US)
Pages (from-to)51-59
Number of pages9
JournalDNA and Cell Biology
Volume11
Issue number1
DOIs
StatePublished - Jan 1992

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