Primary isolation and identification of avian rotaviruses from turkeys exhibiting signs of clinical enteritis in a continuous MA 104 cell line.

S. Y. Kang, K. V. Nagaraja, J. A. Newman

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Avian rotaviruses were isolated from turkeys with enteritis using MA 104 cell line. MA 104 cells were suitable for primary isolation and propagation of avian rotaviruses. Trypsin appeared essential for the enhancement of infectivity and the occurrence of cytopathic effect (CPE). Serum neutralization (SN), electron microscopy (EM), and analysis of genomic RNA were done to identify and confirm the identity of rotaviruses. Electrophoretic migration patterns of genomic RNA from avian rotaviruses were examined, and they were compared with those from mammalian rotaviruses. The migration patterns differed between these groups.

Original languageEnglish (US)
Pages (from-to)494-499
Number of pages6
JournalAvian diseases
Volume30
Issue number3
DOIs
StatePublished - Jan 1 1986

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