Prevalence of influenza a virus (H1N1) antibodies in bovine sera

K. Jones-Lang, M. Ernst-Larson, B. Lee, S. M. Goyal, R. Bey

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

An H1 subtype-specific indirect ELISA was used to determine the prevalence of influenza A virus antibodies in a retrospective study of 2,345 bovine sera in Minnesota. Twenty-seven percent of the samples tested were positive, 31% were low-positive and 42% were negative. The prevalence of antibody appeared to peak during the months of September to November and then again from February to March. A subset of the above samples was examined by hemagglutination-inhibition (HI) to confirm the ELISA results. A 92% correlation was found between the ELISA and hemagglutination-inhibition assay. Western blot analysis on a subset of ELISA positive sera (n=50) confirmed the presence of antibodies to the nucleoprotein and H1 hemagglutinin protein of influenza A virus.

Original languageEnglish (US)
Pages (from-to)153-160
Number of pages8
JournalNew Microbiologica
Volume21
Issue number2
StatePublished - Apr 1 1998

Keywords

  • Bovine H1 subtype
  • ELISA
  • H1N1
  • Influenza
  • Seropositive

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