Deterioration of some complement components occurs during storage at 4 °C. Heat-labile opsonins have been considered identical to certain complement components and it has been assumed that opsonic activity also deteriorates during storage. Fresh and ACD (acid-citrate-dextrose) bank blood were studied for opsonic activity in a bactericidal assay system. Opsonin from fresh ACD blood and fresh leukocytes killed more than 98 per cent of the Staphylococcus aureus and 99 per cent of the Escherichia coli in the bactericidal assay. Opsonin from ACD bank blood stored 21 days supported killing of more than 90 per cent of the S. aureus and 99 per cent of the E. coli. Thus, sufficient opsonic activity remains in ACD bank blood throughout the 21 day shelf life to support killing of these organisms. The transfusion of fresh blood for opsonic activity against S. aureus and E. coli is not necessary.
|Number of pages
|The Journal of laboratory and clinical medicine
|Published - Jun 1972