Presence of a Sexual Population of Puccinia graminis f. Sp. Tritici in Georgia Provides a Hotspot for Genotypic and Phenotypic Diversity

Pablo D. Olivera, Zoya Sikharulidze, Rusudan Dumbadze, Les J. Szabo, Maria Newcomb, Ketino Natsarishvili, Matthew N. Rouse, Douglas G. Luster, Yue Jin

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is a reemerging disease exemplified by recent epidemics caused by new virulent races. Understanding the sources and origins of genetic variations in the pathogen populations globally can facilitate the development of better strategies in disease management. We analyzed 68 wheat stem rust samples collected between 2013 and 2015 from Georgia where stem rust incidences are frequent and the alternate host, common barberry, is present. A total of 116 single-pustule isolates were derived and evaluated on stem rust differential lines to determine the virulence phenotypes and 23 races were identified, many of which were detected for the first time. Unique virulence combinations including, Sr22+Sr24 and Sr13b+Sr35+Sr37 were detected. These virulence combinations pose new challenges to breeding programs because many of these genes are used in breeding for resistance to the Ug99 race group. Sixty-one isolates were genotyped using a custom single-nucleotide polymorphism chip and 17 genotypes were identified. The 2013 isolates contained 11 multilocus genotypes compared with isolates of 2014 and 2015, with five and three genotypes, respectively. The higher levels of virulence and genotypic diversity observed in the 2013 samples strongly indicated that sexual recombination occurs in the Georgian P. graminis f. sp. tritici population, and that the Caucasus region of Eurasia may be an important source of new races.

Original languageEnglish (US)
Pages (from-to)2152-2160
Number of pages9
JournalPhytopathology
Volume109
Issue number12
DOIs
StatePublished - Jan 1 2019

Keywords

  • Ecology and epidemiology
  • Genetics and resistance
  • Genotyping
  • Population biology
  • Race typing
  • Surveillance
  • Wheat stem rust

PubMed: MeSH publication types

  • Journal Article

Fingerprint Dive into the research topics of 'Presence of a Sexual Population of Puccinia graminis f. Sp. Tritici in Georgia Provides a Hotspot for Genotypic and Phenotypic Diversity'. Together they form a unique fingerprint.

  • Cite this