Abstract
Alginate lyase is an enzyme that catalyses the hydrolysis of alginate into alginate oligoalginates. To enhance enzyme stability and recovery, a facile strategy for alginate lyase immobilization was developed. Novel magnetic chitosan microspheres were synthesized and used as carriers to immobilize alginate lyase. The immobilization of alginate lyase on magnetic chitosan microspheres was successful, as proven by Fourier transform infrared spectroscopy and X-ray diffraction spectra. Enzyme immobilization exhibited the best performance at an MCM dosage of 1.5 g/L, adsorption time of 2.0 h, glutaraldehyde concentration of 0.2%, and immobilization time of 2.0 h. The optimal pH of the free alginate lyase was 7.5, and this pH value was shifted to 8.0 after immobilization. No difference was observed at the optimal temperature (45 °C) for the immobilized and free enzymes. The immobilized alginate lyase displayed better thermal stability than the free alginate lyase. The Km values of the free and immobilized enzymes were 0.05 mol/L and 0.09 mol/L, respectively. The immobilized alginate lyase retained 72% of its original activity after 10 batch reactions. This strategy was found to be a promising method for immobilizing alginate lyase.
Original language | English (US) |
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Pages (from-to) | 5403-5418 |
Number of pages | 16 |
Journal | Applied Biochemistry and Biotechnology |
Volume | 196 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2024 |
Bibliographical note
Publisher Copyright:© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023.
Keywords
- Alginate lyase
- Enzyme recovery
- Enzyme stability
- Immobilization
- Magnetic chitosan microspheres