Preparation, purification, and use of fatty acid-containing liposomes

Lin Jin, Aaron E. Engelhart, Katarzyna P. Adamala, Jack W. Szostak

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of these amphiphiles. In particular, fatty acid liposomes enhance dynamic character, due to the relatively high solubility of single-chain amphiphiles. Similarly, liposomes containing free fatty acids are more sensitive to salt and divalent cations, due to the strong interactions between the carboxylic acid head groups and metal ions. Here we illustrate techniques for preparation, purification, and use of liposomes comprised in part or whole of single chain amphiphiles (e.g., oleic acids).

Original languageEnglish (US)
Article numbere57324
JournalJournal of Visualized Experiments
Issue number132
StatePublished - Feb 9 2018

Bibliographical note

Funding Information:
J.W.S. is an Investigator of the Howard Hughes Medical Institute. This work was supported in part by a grant (290363) from the Simons Foundation to J.W.S. Both A.E.E. and K.P.A. acknowledge support from University of Minnesota startup funds.


  • Bioengineering
  • Issue 132
  • Liposomes
  • Origin of life
  • Protocells
  • Synells
  • Synthetic cells
  • Vesicles

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