Preparation of individual galactan oligomers, their prebiotic effects, and use in estimating galactan chain length in pectin-derived polysaccharides

Yi Zheng, Leisheng Li, Zhangkai Feng, Hao Wang, Kevin H. Mayo, Yifa Zhou, Guihua Tai

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Galactans exhibit many biological activities, including modulation of human gut microbiota. By optimizing hydrolysis conditions and fractionation protocols, we purified a series of galacto-oligosaccharides from potato galactan, with degrees of polymerization (DP) up to 15. These oligosaccharides were characterized by using HPAEC, MALDI-TOF, 1H13C-NMR HSQC and HMBC experiments. Analysis of NMR data allowed chemical shift assignments to be made for oligos up to DP15. We used these NMR data to calculate intensity ratios of resonances from non-terminal to terminal residues, and correlated them with galactan chain length. Using this approach, we were able to estimate the length of galactan side chains in complex rhamnogalacturonan-I polysaccharides. Furthermore, we demonstrate here that prebiotic effects on Lactobacillus bacteria are related to galactan chain length, suggesting a potential application in the study of the human gut microbiome.

Original languageEnglish (US)
Pages (from-to)526-533
Number of pages8
JournalCarbohydrate Polymers
Volume199
DOIs
StatePublished - Nov 1 2018

Keywords

  • 2D NMR
  • Galactan oligosaccharides
  • MS
  • Prebiotics
  • Structural analysis

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