Preliminary crystallographic analysis of methane mono-oxygenase hydroxylase from Methylosinus trichosporium OB3b

Wayne A. Froland; David H. Dyer; R. Radhakrishnan; Cathleen A. Earhart; John D. Lipscomb; Douglas H. Ohlendorf

doi:10.1006/jmbi.1994.1145

Preliminary crystallographic analysis of methane mono-oxygenase hydroxylase from Methylosinus trichosporium OB3b

Wayne A. Froland
, David H. Dyer
, R. Radhakrishnan
, Cathleen A. Earhart
, John D. Lipscomb
, Douglas H. Ohlendorf

Biochemistry, Molecular Biology, and Biophysics (TMED)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The hydroxylase component of the enzyme methane mono-oxygenase from Methylosinus trichosporium OB3b has been crystallized in the orthorhombic space group C222₁ with unit cell dimensions a = 264.5 Å, b = 71.2 Å, c = 139.4 Å. The crystals grow as square, thick plates and diffract to beyond 2 Å resolution. There is one half of the hydroxylase dimer in the asymmetric unit.

Original language	English (US)
Pages (from-to)	379-381
Number of pages	3
Journal	Journal of Molecular Biology
Volume	236
Issue number	1
DOIs	https://doi.org/10.1006/jmbi.1994.1145
State	Published - Feb 10 1994

Keywords

Crystallization
Hydroxylases
Methylosinus trichosporium OB3b
Oxygenases
X-ray crystallography

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10.1006/jmbi.1994.1145

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title = "Preliminary crystallographic analysis of methane mono-oxygenase hydroxylase from Methylosinus trichosporium OB3b",

abstract = "The hydroxylase component of the enzyme methane mono-oxygenase from Methylosinus trichosporium OB3b has been crystallized in the orthorhombic space group C2221 with unit cell dimensions a = 264.5 {\AA}, b = 71.2 {\AA}, c = 139.4 {\AA}. The crystals grow as square, thick plates and diffract to beyond 2 {\AA} resolution. There is one half of the hydroxylase dimer in the asymmetric unit.",

keywords = "Crystallization, Hydroxylases, Methylosinus trichosporium OB3b, Oxygenases, X-ray crystallography",

author = "Froland, \{Wayne A.\} and Dyer, \{David H.\} and R. Radhakrishnan and Earhart, \{Cathleen A.\} and Lipscomb, \{John D.\} and Ohlendorf, \{Douglas H.\}",

year = "1994",

month = feb,

day = "10",

doi = "10.1006/jmbi.1994.1145",

language = "English (US)",

volume = "236",

pages = "379--381",

journal = "Journal of Molecular Biology",

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T1 - Preliminary crystallographic analysis of methane mono-oxygenase hydroxylase from Methylosinus trichosporium OB3b

AU - Froland, Wayne A.

AU - Dyer, David H.

AU - Radhakrishnan, R.

AU - Earhart, Cathleen A.

AU - Lipscomb, John D.

AU - Ohlendorf, Douglas H.

PY - 1994/2/10

Y1 - 1994/2/10

N2 - The hydroxylase component of the enzyme methane mono-oxygenase from Methylosinus trichosporium OB3b has been crystallized in the orthorhombic space group C2221 with unit cell dimensions a = 264.5 Å, b = 71.2 Å, c = 139.4 Å. The crystals grow as square, thick plates and diffract to beyond 2 Å resolution. There is one half of the hydroxylase dimer in the asymmetric unit.

AB - The hydroxylase component of the enzyme methane mono-oxygenase from Methylosinus trichosporium OB3b has been crystallized in the orthorhombic space group C2221 with unit cell dimensions a = 264.5 Å, b = 71.2 Å, c = 139.4 Å. The crystals grow as square, thick plates and diffract to beyond 2 Å resolution. There is one half of the hydroxylase dimer in the asymmetric unit.

KW - Crystallization

KW - Hydroxylases

KW - Methylosinus trichosporium OB3b

KW - Oxygenases

KW - X-ray crystallography

UR - https://www.scopus.com/pages/publications/0028327710

UR - https://www.scopus.com/pages/publications/0028327710#tab=citedBy

U2 - 10.1006/jmbi.1994.1145

DO - 10.1006/jmbi.1994.1145

M3 - Article

C2 - 8107121

AN - SCOPUS:0028327710

SN - 0022-2836

VL - 236

SP - 379

EP - 381

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Preliminary crystallographic analysis of methane mono-oxygenase hydroxylase from Methylosinus trichosporium OB3b

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