Preferential accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in breast cancer: A comprehensive study on six breast cell lines with varying phenotypes

Stacy R. Millon, Julie H. Ostrander, Siavash Yazdanfar, J. Quincy Brown, Janelle E. Bender, Anita Rajeha, Nirmala Ramanujam

Research output: Contribution to journalArticle

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Abstract

We describe the potential of 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) fluorescence as a source of contrast for margin detection in commonly diagnosed breast cancer subtypes. Fluorescence intensity of PpIX in untreated and ALA-treated normal mammary epithelial and breast cancer cell lines of varying estrogen receptor expression were quantitatively imaged with confocal microscopy. Percentage change in fluorescence intensity integrated over 610-700 nm (attributed to PpIX) of posttreated compared to pretreated cells showed statistically significant differences between four breast cancer and two normal mammary epithelial cell lines. However, a direct comparison of post-treatment PpIX fluorescence intensities showed no differences between breast cancer and normal mammary epithelial cell lines due to confounding effects by endogenous fluorescence from flavin adenine dinucleotide (FAD). Clinically, it is impractical to obtain pre-and post-treatment images. Thus, spectral imaging was demonstrated as a means to remove the effects of endogenous FAD fluorescence allowing for discrimination between posttreatment PpIX fluorescence of four breast cancer and two normal mammary epithelial cell lines. Fluorescence spectral imaging of ALA-treated breast cancer cells showed preferential PpIX accumulation regardless of malignant phenotype and suggests a useful contrast mechanism for discrimination of residual cancer at the surface of breast tumor margins.

Original languageEnglish (US)
Article number018002
JournalJournal of biomedical optics
Volume15
Issue number1
DOIs
StatePublished - Dec 1 2010

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Aminolevulinic Acid
phenotype
cultured cells
breast
cancer
Fluorescence
Cells
fluorescence
acids
Acids
Flavin-Adenine Dinucleotide
adenines
discrimination
margins
estrogens
Imaging techniques
protoporphyrin IX
Confocal microscopy
Estrogen Receptors
Tumors

Keywords

  • 5-Aminolevulinic acid
  • Breast cells
  • Confocal microscopy
  • Fluorescence intensity
  • Protoporphyrin IX
  • Spectroscopy

Cite this

Preferential accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in breast cancer : A comprehensive study on six breast cell lines with varying phenotypes. / Millon, Stacy R.; Ostrander, Julie H.; Yazdanfar, Siavash; Quincy Brown, J.; Bender, Janelle E.; Rajeha, Anita; Ramanujam, Nirmala.

In: Journal of biomedical optics, Vol. 15, No. 1, 018002, 01.12.2010.

Research output: Contribution to journalArticle

Millon, Stacy R. ; Ostrander, Julie H. ; Yazdanfar, Siavash ; Quincy Brown, J. ; Bender, Janelle E. ; Rajeha, Anita ; Ramanujam, Nirmala. / Preferential accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in breast cancer : A comprehensive study on six breast cell lines with varying phenotypes. In: Journal of biomedical optics. 2010 ; Vol. 15, No. 1.
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