The primary purpose of this study was to determine the accuracy of estimating oxygen uptake (V̇O2) from the flywheel revolution rate of a bicycle wind-loaded simulator. V̇O2 at four different flywhell revolution rates was measured on a Findlay Road Machine (FRM). Ten male trained cyclists, 10 male untrained cyclists, 10 female trained cyclists and 10 female untrained cyclists served as subjects. Significant curvilinear relationships (P < 0.01) were found between road speed estimated from flywheel revolution rate and V̇O2 expressed as l.min-1, ml.kg-1 .min-1, l.min-1.m-2 (r = 0.97, 0.96, 0.98, respectively). The absolute standard error of the mean V̇O2 was 0.21 l.min-1 (9.6%), 3.71 ml.kg-1.min-1 (11.5%) and 0.10 l.min-1.m-2 (7.9%), respectively. The relationship between V̇O2 and speed was similar to that reported during road cycling. To determine the magnitude of between-machine differences in V̇O2, six subjects randomly performed cycling using two different FMR. Significant (P < 0.05) differences between machines were found at only the highest speed. The present study indicates that it is possible to accurately predict V̇O2 from flywheel revolution rate using a FRM. Since the FRM Since the FRM appears to approximate the resistance a cyclist experiences on the raod and allows cyclists to use their own bicycle, it provides a good alternative to traditional laboratory ergometers.