Predicting the in vivo accumulation of nanoparticles in tumor based on in vitro macrophage uptake and circulation in zebrafish

Hyeyoun Chang, Ji Young Yhee, Gun Hyuk Jang, Dong Gil You, Ju Hee Ryu, Yongwhan Choi, Jin Hee Na, Jae Hyung Park, Kwan Hyi Lee, Kuiwon Choi, Kwangmeyung Kim, Ick Chan Kwon

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Nanoparticles have resulted in great progress in biomedical imaging and targeted drug delivery in cancer theranostics. To develop nanoparticles as an effective carrier system for therapeutics, chemical structures and physicochemical properties of nanoparticle may provide a reliable means to predict the in vitro characteristics of nanoparticles. However, in vivo fates of nanoparticles, such as pharmacokinetics and tumor targeting efficiency of nanoparticles, have been difficult to predict beforehand. To predict the in vivo fates of nanoparticles in tumor-bearing mice, differences in physicochemical properties and in vitro cancer cell/macrophage uptake of 5 different nanoparticles with mean diameter of 200–250 nm were comparatively analyzed, along with their circulation in adult zebrafish. The nanoparticles which showed favorable cellular uptake by macrophages indicated high unintended liver accumulation in vivo, which is attributed to the clearance by the reticuloendothelial system (RES). In addition, blood circulation of nanoparticles was closely correlated in adult zebrafish and in mice that the zebrafish experiment may elucidate the in vivo behavior of nanoparticles in advance of the in vivo experiment using mammal animal models. This comparative study on various nanoparticles was conducted to provide the basic information on predicting the in vivo fates of nanoparticles prior to the in vivo experiments.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalJournal of Controlled Release
StatePublished - Dec 28 2016

Bibliographical note

Funding Information:
This study was funded by the Global Research Laboratory Project ( NRF-2013K1A1A2A02050115 ) of Korea National Research Fund (KNRF), Nano-Convergence Foundation funded by the Ministry of Science, ICT and Future Planning , and Development and Commercialization of Nanoparticle Sensor for Detecting Protease Activity Project by the Ministry of Trade, Industry and Energy (MOTIE, Korea), and KIST intramural Fund (Theragnosis).

Publisher Copyright:
© 2016 Elsevier B.V.

Copyright 2017 Elsevier B.V., All rights reserved.


  • Macrophage
  • Nanoparticle
  • Reticuloendothelial system
  • Targeting
  • Tumor


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