Precision Targeted Ablation of Fine Neurovascular Structures In Vivo Using Dual-mode Ultrasound Arrays

Raj Aravalli, Dusty Van Helden, Dalong Liu, Parker O’Brien, Hasan Aldiabat, Alexandru-Flaviu Tabaran, M. Gerard O’Sullivan, H. Brent Clark, John W. Osborn, Emad S. Ebbini

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Carotid bodies (CBs) are chemoreceptors that monitor and register changes in the blood, including the levels of oxygen, carbon dioxide, and pH, and regulate breathing. Enhanced activity of CBs was shown to correlate with a significant elevation in the blood pressure of patients with hypertension. CB removal or denervation were previously shown to reduce hypertension. Here we demonstrate the feasibility of a dual-mode ultrasound array (DMUA) system to safely ablate the CB in vivo in a spontaneously hypertensive rat (SHR) model of hypertension. DMUA imaging was used for guiding and monitoring focused ultrasound (FUS) energy delivered to the target region. In particular, 3D imaging was used to identify the carotid bifurcation for targeting the CBs. Intermittent, high frame rate imaging during image-guided FUS (IgFUS) delivery was used for monitoring the lesion formation. DMUA imaging provided feedback for closed-loop control (CLC) of the lesion formation process to avoid overexposure. The procedure was tolerated well in over 100 SHR and normotensive rats that received unilateral and bilateral treatments. The measured mean arterial pressure (MAP) exhibited measurable deviation from baseline 2–4 weeks post IgFUS treatment. The results suggest that the direct unilateral FUS treatment of the CB might be sufficient to reduce the blood pressure in hypertensive rats and justify further investigation in large animals and eventually in human patients.

Original languageEnglish (US)
Article number9249
JournalScientific reports
Volume10
Issue number1
DOIs
StatePublished - Jun 8 2020

Bibliographical note

Funding Information:
Authors thank Colleen Forster for help with histology, and Meri DuRand for animal monitoring. This work was supported by funding from International Cardio Corporation.

Publisher Copyright:
© 2020, The Author(s).

Keywords

  • Animals
  • Carotid Body/pathology
  • High-Intensity Focused Ultrasound Ablation/instrumentation
  • Hypertension/diagnostic imaging
  • Male
  • Rats
  • Rats, Inbred SHR
  • Surgery, Computer-Assisted/instrumentation
  • Vital Signs

PubMed: MeSH publication types

  • Journal Article

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