Precipitation of calcium gluceptate from aqueous solutions

Raj Suryanarayanan, A. G. Mitchell

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A precipitate encountered in solutions of calcium gluceptate was identified as hydrated calcium gluceptate. Precipitation was associated with a change from a very soluble amorphous anhydrous form to a sparingly soluble crystalline hydrate, the presence of seed crystals inducing crystallization, and unsuitable proportions of the α‐ and β‐epimers of calcium gluceptate. Various commercial samples and the corresponding precipitates were examined by elemental analysis, thermal analysis, X‐ray diffraction, IR spectroscopy, and GC‐MS. The proportion of the α‐ and β‐epimers in commercial samples was quantitated by GC. In this method, an aqueous solution of calcium gluceptate was converted into a mixture of glucoheptonic acids and their corresponding lactones by passage through a cation‐exchange resin. The solution was freeze‐dried, the acid‐lactone mixture converted to the γ‐lactones using concentrated hydrochloric acid, and the resulting material trimethylsilylated with trimethylsilylimidazole. Stability studies of solutions prepared from calcium gluceptate obtained from various commercial sources indicate that above ˜50% α‐epimer, stability decreased with an increase in the relative proportion of the α‐epimer. Material complying with USP specifications (pure α‐epimer) is the least stable in solution. It is suggested that calcium gluceptate containing approximately equal proportions of the α‐ and β‐epimers be introduced in the USP monograph together with a method for estimating the proportions of the epimers.

Original languageEnglish (US)
Pages (from-to)78-82
Number of pages5
JournalJournal of Pharmaceutical Sciences
Volume73
Issue number1
DOIs
StatePublished - Jan 1984
Externally publishedYes

Keywords

  • Calcium gluceptate—precipitation in aqueous solutions, identification of α‐ and β‐epimers, quantitation by GC
  • Epimers—calcium gluceptate, quantitation by GC, role in precipitation in aqueous solutions
  • Precipitation—calcium gluceptate in aqueous solutions, role of epimers, quantitation by GC

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