Abstract
We describe a novel RNA binding protein, Y14, a predominantly nuclear nucleocytoplasmic shuttling protein. Interestingly, Y14 associates preferentially with mRNAs produced by splicing but not with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Y14 associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. Splicing of a single intron is sufficient for Y14 association. Y14-containing nuclear complexes are different from general hnRNP complexes. They contain hnRNP proteins and several unique proteins including the mRNA export factor TAP. Thus, Y14 defines novel intermediate in the pathway of gene expression, postsplicing nuclear preexport mRNPs, and newly exported cytoplasmic mRNPs, whose composition is established by splicing. These findings suggest that pre-mRNA splicing imprints mRNA with a unique set of proteins that persists in the cytoplasm and thereby communicates the history of the transcript.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 673-682 |
| Number of pages | 10 |
| Journal | Molecular Cell |
| Volume | 6 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 2000 |
| Externally published | Yes |
Bibliographical note
Funding Information:We are grateful to Drs. Adrian Krainer, Elisa Izaurralde, and Iain Mattaj for kindly providing antibodies and plasmids. We are grateful to members of our laboratory, especially Sara Nakielny, Westley Friesen, Zissimos Mourelatos, Lili Wan, and Chia Chan for critical reading of and comments on this manuscript. We also thank Sue Kelchner for secretarial assistance. This work was supported by grants from the National Institutes of Health and the Human Frontier Science Program Organization. G. D. is an Investigator of the Howard Hughes Medical Institute.