@inbook{7fc377608ccd43798634cb3e852c6e32,
title = "Practical methods for in vivo cortical physiology with 2-photon microscopy and bulk loading of fluorescent calcium indicator dyes",
abstract = "In vivo 2-photon imaging of neurons that have been bulk-loaded with fl uorescent calcium indicator dyes is permitting many fundamental principles of neural circuit organization and development to be uncovered. In this article, we describe the materials and procedures that we have used in our investigations of ferrets, tree shrews, and mice. Special attention is given to the design and construction of custom stereotaxic devices and the prevention of stray light from entering the 2-photon microscope during vision experiments.",
keywords = "2-photon, AM calcium dyes, AM dyes, Epifluorescence, Head plate, Headplate, Light block, Optical chamber, Oregon Green BAPTA-1, Two-photon",
author = "Hooser, {Stephen D.Van} and Johnson, {Elizabeth N.} and Ye Li and Mark Mazurek and Culp, {Julie H.} and Arani Roy and Rishabh Kasliwal and Kelly Flavahan",
year = "2015",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-1963-5_6",
language = "English (US)",
isbn = "9781493919628",
volume = "92",
series = "Neuromethods",
publisher = "Springer New York",
pages = "117--141",
booktitle = "Neural Tracing Methods",
}