Practical laboratory exercises for plant molecular cytogenetics

Scott A. Jackson; Shahryar F. Kianian; Khwaja G. Hossain; Jason G. Walling

doi:10.1007/978-0-387-70869-0_15

Practical laboratory exercises for plant molecular cytogenetics

Scott A. Jackson, Shahryar F. Kianian, Khwaja G. Hossain, Jason G. Walling

Plant Pathology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

Practical laboratory work parallel with a lecture topic is one way to improve understanding of the subject matter. Cytogenetics deals mainly with chromosome behavior and biology. The key concepts of cytogenetics can be attached to actual experimental observations that can help students visualize the relationship of the concepts to chromosomes in practice. Many new technological developments are available to aid understanding of chromosome behavior, biology, and manipulation for plant and animal improvement. We present here a series of exercises designed to help students understand chromosome behavior during cellular divisions, chromosomal aberrations, and chromosome preparations for molecular cytogenetics, such as fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), and even fiber-FISH. These exercises are used in several undergraduate and graduate courses. If they are to be incorporated into a cytogenetics course, we recommend that the instructor choose those that fit the course time-table and available resources.

Original language	English (US)
Title of host publication	Plant Cytogenetics
Subtitle of host publication	Genome Structure and Chromosome Function
Publisher	Springer New York
Pages	323-333
Number of pages	11
Volume	9
ISBN (Electronic)	9780387708690
ISBN (Print)	9780387708683
DOIs	https://doi.org/10.1007/978-0-387-70869-0_15
State	Published - Jan 1 2012

Keywords

Aneuploidy
Deletion line
EST
QTL
Radiation hybrid
SSR
Substitution line

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abstract = "Practical laboratory work parallel with a lecture topic is one way to improve understanding of the subject matter. Cytogenetics deals mainly with chromosome behavior and biology. The key concepts of cytogenetics can be attached to actual experimental observations that can help students visualize the relationship of the concepts to chromosomes in practice. Many new technological developments are available to aid understanding of chromosome behavior, biology, and manipulation for plant and animal improvement. We present here a series of exercises designed to help students understand chromosome behavior during cellular divisions, chromosomal aberrations, and chromosome preparations for molecular cytogenetics, such as fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), and even fiber-FISH. These exercises are used in several undergraduate and graduate courses. If they are to be incorporated into a cytogenetics course, we recommend that the instructor choose those that fit the course time-table and available resources.",

keywords = "Aneuploidy, Deletion line, EST, QTL, Radiation hybrid, SSR, Substitution line",

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AU - Kianian, Shahryar F.

AU - Hossain, Khwaja G.

AU - Walling, Jason G.

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KW - Deletion line

KW - EST

KW - QTL

KW - Radiation hybrid

KW - SSR

KW - Substitution line

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ER -

Practical laboratory exercises for plant molecular cytogenetics

Abstract

Keywords

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