Practical laboratory exercises for plant molecular cytogenetics

Scott A. Jackson, Shahryar F. Kianian, Khwaja G. Hossain, Jason G. Walling

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Practical laboratory work parallel with a lecture topic is one way to improve understanding of the subject matter. Cytogenetics deals mainly with chromosome behavior and biology. The key concepts of cytogenetics can be attached to actual experimental observations that can help students visualize the relationship of the concepts to chromosomes in practice. Many new technological developments are available to aid understanding of chromosome behavior, biology, and manipulation for plant and animal improvement. We present here a series of exercises designed to help students understand chromosome behavior during cellular divisions, chromosomal aberrations, and chromosome preparations for molecular cytogenetics, such as fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH), and even fiber-FISH. These exercises are used in several undergraduate and graduate courses. If they are to be incorporated into a cytogenetics course, we recommend that the instructor choose those that fit the course time-table and available resources.

Original languageEnglish (US)
Title of host publicationPlant Cytogenetics
Subtitle of host publicationGenome Structure and Chromosome Function
PublisherSpringer New York
Pages323-333
Number of pages11
Volume9
ISBN (Electronic)9780387708690
ISBN (Print)9780387708683
DOIs
StatePublished - Jan 1 2012

Keywords

  • Aneuploidy
  • Deletion line
  • EST
  • QTL
  • Radiation hybrid
  • SSR
  • Substitution line

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