PR-b functionalized stealth liposomes for targeted delivery to metastatic colon cancer

Maroof Adil, Lalitha Belur, Timothy R. Pearce, Rachel M. Levine, Alison W. Tisdale, Brent S. Sorenson, R S Mc Ivor, Efrosini Kokkoli

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A gene delivery system was designed to carry a payload to integrin overexpressing cells. Branched-polyethyleneimine (bPEI) condensed plasmid DNA was encapsulated into targeted stealth liposomes, thereby combining the condensing and transfection properties of bPEI with the stealth and targeting properties of the liposomal carrier system. PR-b was used as a targeting ligand-a peptide we designed to bind specifically to the cancer cell surface marker α5β1 integrin-and such a robust receptor-ligand interaction achieved higher specificity than what has been previously reported for targeted delivery systems. In the process of formulating the PR-b functionalized gene delivery vehicle, we developed a protocol to fully encapsulate condensed DNA in liposomes and accurately quantify the total DNA in the system. We demonstrate that compared to non-targeted stealth liposomes and non-encapsulated condensed DNA, the PR-b functionalized stealth liposomes mediated improved in vitro transfection specifically to colon cancer cells overexpressing the α5β1 integrin. Furthermore, when administered in vivo to metastatic tumor bearing mice, PR-b functionalized stealth liposomes outperformed non-targeted liposomes and delivered genes specifically to the tumor site.

Original languageEnglish (US)
Pages (from-to)393-401
Number of pages9
JournalBiomaterials Science
Volume1
Issue number4
DOIs
StatePublished - Apr 1 2013

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Liposomes
DNA
Integrins
Polyethyleneimine
Genes
Tumors
Bearings (structural)
Ligands
Cells
Peptides
Plasmids

Cite this

Adil, M., Belur, L., Pearce, T. R., Levine, R. M., Tisdale, A. W., Sorenson, B. S., ... Kokkoli, E. (2013). PR-b functionalized stealth liposomes for targeted delivery to metastatic colon cancer. Biomaterials Science, 1(4), 393-401. https://doi.org/10.1039/c2bm00128d

PR-b functionalized stealth liposomes for targeted delivery to metastatic colon cancer. / Adil, Maroof; Belur, Lalitha; Pearce, Timothy R.; Levine, Rachel M.; Tisdale, Alison W.; Sorenson, Brent S.; Mc Ivor, R S; Kokkoli, Efrosini.

In: Biomaterials Science, Vol. 1, No. 4, 01.04.2013, p. 393-401.

Research output: Contribution to journalArticle

Adil, M, Belur, L, Pearce, TR, Levine, RM, Tisdale, AW, Sorenson, BS, Mc Ivor, RS & Kokkoli, E 2013, 'PR-b functionalized stealth liposomes for targeted delivery to metastatic colon cancer', Biomaterials Science, vol. 1, no. 4, pp. 393-401. https://doi.org/10.1039/c2bm00128d
Adil, Maroof ; Belur, Lalitha ; Pearce, Timothy R. ; Levine, Rachel M. ; Tisdale, Alison W. ; Sorenson, Brent S. ; Mc Ivor, R S ; Kokkoli, Efrosini. / PR-b functionalized stealth liposomes for targeted delivery to metastatic colon cancer. In: Biomaterials Science. 2013 ; Vol. 1, No. 4. pp. 393-401.
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